Separation of Sperm by Micromanipulator from Unusual Forensic Sample – case report

Česká verzia

Authors: David Vlček ¹; Dan Vaněk ^2,3; Jaromír Mikeš ⁴; Martin Kováč ⁵; Dalibor Kállay ⁵
Authors place of work: Kriminalistický a expertizní ústav PZ Košice ¹; Forenzní DNA servis, Praha ²; 2. lékařská fakulta UK, Praha ³; Ústav biologických a ekologických věd, Prírodovedecká fakulta, upjš Košice ⁴; Soudně-lékařské a patologicko-anatomické pracoviště ÚDZS Prešov ⁵
Published in the journal: Soud Lék., 58, 2013, No. 2, p. 16-18
Category: Původní práce

Summary

The aim of this study is to provide an information about the method of processing of unusual forensic sample that was submitted for the sexual assault case. We analyzed microscopic sample of vaginal swab stained using the hematoxylin-eosin mixture. After removing the covering glass we failed to collect the sperm cells to the micromanipulator capillary. The cells even started, due to the mechanical stress, to fall apart. We think that the main reason of the microdissection failure is the advanced cell lysis. Due to the negative results of the DNA analysis we defined a set of preventive and corrective actions that would (in case of application) increase the chance of positive identification in similar sexual assault cases.

Keywords:
Forensic – microdissection – DNA identification – sampling – sexual assault

Zdroje

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