Assisted oocyte activation

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Authors: P. Trávník ^1-3 ; M Ješeta ^1,4,5 ; R. Hűttelová ^1,6 ; R. Křen ^1,7 ; L. Landsmann ^1,8 ; A. Nesvadbová ^1,9,10 ; G. Tauwinklová ^1,2,11
Authors place of work: Výbor Asociace reprodukční embryologie z. s. ¹; Repromeda s. r. o., Brno ²; IPVZ, Praha ³; Gynekologicko-porodnická klinika LF MU a FN Brno ⁴; FAPPZ ČZU, Praha ⁵; IVF Cube SE, Praha ⁶; GENNET s. r. o., Praha ⁷; UNICA Prague s. r. o., Praha ⁸; IVF Clinic a. s., Olomouc ⁹; Přírodovědecká fakulta UP, Olomouc ¹⁰; Ústav histologie a embryologie, LF MU, Brno ¹¹
Published in the journal: Ceska Gynekol 2023; 88(6): 459-462
Category:

Summary

Objective: Currently, there is a rapid increase in studies on assisted oocyte activation, which can significantly improve the process of in vitro fertilization. Fertilization of oocytes by conventional methods and by intracytoplasmic sperm injection can be affected by insufficient activation of the oocyte. The reason is mainly deviations in the enzymatic equipment of sperm or oocytes or a non-functional activation cascade. In many cases, fertilization can be achieved using artificial oocyte activation by applying calcium ion donors to the oocytes after sperm microinjection. However, opinions on the safety and reliability of this method are not uniform. The aim of the thesis is to present current knowledge about assisted oocyte activation and its impact not only on in vitro fertilization, but also on subsequent embryonic and fetal development. Methodology: Research of relevant literature in Web of Science, PubMed/Medline and Scopus databases. Results and conclusions: Based on the literature data and the authors’ own experience, it follows that this method is effective and safe from the point of view of further development of the embryo, fetus and postnatal development. Extensive meta-analyses focused on this method were carried out, which did not find a negative impact not only on the embryonic and fetal development of the individual, but this method did not have associated with a negative impact on the psychosomatic development of the children.

Zdroje

1. Vanden Meerschaut F, Nikiforaki D, Heindryckx B et al. Assisted oocyte activation following ICSI fertilization failure. Reprod Biomed Online 2014; 28 (5): 560–571. doi: 10.1016/ j.rbmo.2014.01.008.

2. Tesarik J, Rienzi L, Ubaldi F et al. Use of a modified intracytoplasmic sperm injection technique to overcome sperm-borne and oocyte-borne oocyte activation failures. Fertil Steril 2002; 78 (3): 619–624. doi: 10.1016/s0015-0282 (02) 03 291-0.

3. Hoshi K, Yanagida K, Yazawa H et al. Intracytoplasmic sperm injection using immobilized or motile human spermatozoon. Fertil Steril 1995; 63 (6): 1241–1245. doi: 10.1016/00 20-7292 (96) 88083-0.

4. Murugesu S, Saso S, Jones BP et al. Does the use of calcium ionophore during artificial oocyte activation demonstrate an effect on pregnancy rate? A meta-analysis. Fertil Steril 2017; 108 (3): 468.e3–482.e3. doi: 10.1016/j.fertnstert. 2017.06.029.

5. Bridges PJ, Jeoung M, Kim H et al. Methodology matters: IVF versus ICSI and embryonic gene expression. Reprod Biomed Online 2011; 23 (2): 234–244. doi: 10.1016/j.rbmo.2011.04.007.

6. Ferrer-Buitrago M, Tilleman L, Thys V et al. Comparative study of preimplantation development following distinct assisted oocyte activation protocols in a PLC-zeta knockout mouse model. Mol Hum Reprod 2020; 26 (11): 801–815. doi: 10.1093/molehr/gaaa060.

7. Zhang J, Yao G, Zhang T et al. Effect of calcium ionophore (A23187) on embryo development and its safety in PGT cycles. Front Endocrinol (Lausanne) 2023; 13: 979248. doi: 10.3389/fendo.2022.979248.

8. Miller N, Biron-Shental T, Sukenik-Halevy R et al. Oocyte activation by calcium ionophore and congenital birth defects: a retrospective cohort study. Fertil Steril 2016; 106 (3): 590.e2–596.e2. doi: 10.1016/j.fertnstert.2016.04.025.

9. Yoon HJ, Bae IH, Kim HJ et al. Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore. J Assist Reprod Genet 2013; 30 (12): 1569–1575. doi: 10.1007/s10815-013-0110-2.

10. Deemeh MR, Tavalaee M, Nasr-Esfahani MH. Health of children born through artificial oocyte activation: a pilot study. Reprod Sci 2015; 22 (3): 322–328. doi: 10.1177/1933719114542017.

11. Li B, Zhou Y, Yan Z et al. Pregnancy and neonatal outcomes of artificial oocyte activation in patients undergoing frozen-thawed embryo transfer: a 6-year population-based retrospective study. Arch Gynecol Obstet 2019; 300 (4): 1083–1092. doi: 10.1007/s00404-019-052 98-3.

12. Long R, Wang M, Yang QY et al. Risk of birth defects in children conceived by artificial oocyte activation and intracytoplasmic sperm injection: a meta-analysis. Reprod Biol Endocrinol 2020; 18 (1): 123. doi: 10.1186/s12958-020-006 80-2.

13. Shan Y, Zhao H, Zhao D et al. Assisted oocyte activation with calcium ionophore improves pregnancy outcomes and offspring safety in infertile patients: a systematic review and meta-analysis. Front Physiol 2022; 12: 751905. doi: 10.3389/fphys.2021.751905.

14. Morgan HD, Santos F, Green K et al. Epigenetic reprogramming in mammals. Hum Mol Genet 2005; 14 (1): R47–R58. doi: 10.1093/hmg/ddi114.

15. Zafar MI, Lu S, Li H. Sperm-oocyte interplay: an overview of spermatozoon’s role in oocyte activation and current perspectives in diagnosis and fertility treatment. Cell Biosci 2021; 11 (1): 4. doi: 10.1186/s13578-020-00520-1.

16. Martínez M, Durban M, Santaló J et al. Assisted oocyte activation effects on the morphokinetic pattern of derived embryos. J Assist Reprod Genet 2021; 38 (2): 531–537. doi: 10.1007/s10815-020-02025-9.

17. Niu X, Ruan Q, Witz CA et al. Comparison of human oocyte activation between round-headed sperm injection followed by calcium ionophore treatment and normal sperm injection in a patient with globozoospermia. Front Endocrinol (Lausanne) 2020; 11: 183. doi: 10.3389/fendo.2020.00183.

18. Kang HJ, Lee SH, Park YS et al. Artificial oocyte activation in intracytoplasmic sperm injection cycles using testicular sperm in human in vitro fertilization. Clin Exp Reprod Med 2015; 42 (2): 45–50. doi: 10.5653/cerm.2015.42. 2.45.

19. Alvarez Sedó C, Rawe VY, Chemes HE. Acrosomal bio genesis in human globozoospermia: immunocytochemical, ultrastructural and proteomic studies. Hum Reprod 2012; 27 (7): 1912–1921. doi: 10.1093/humrep/des126.

20. Bechoua S, Chiron A, Delcleve-Paulhac S et al. Fertilisation and pregnancy outcome after ICSI in globozoospermic patients without assisted oocyte activation. Andrologia 2009; 41 (1): 55–58. doi: 10.1111/j.1439-0272.2008.008 84.x.

21. Ebner T, Maurer M, Oppelt P et al. Healthy twin live-birth after ionophore treatment in a case of theophylline-resistant Kartagener syndrome. J Assist Reprod Genet 2015; 32 (6): 873–877. doi: 10.1007/s10815-015-0486-2.

22. Economou KA, Christopikou D, Tsorva E et al. The combination of calcium ionophore A23187 and GM-CSF can safely salvage aged human unfertilized oocytes after ICSI. J Assist Reprod Genet 2017; 34 (1): 33–41. doi: 10.1007/s10815-016-0823-0.

23. Xu Z, Yao G, Niu W et al. Calcium ionophore (A23187) rescues the activation of unfertilized oocytes after intracytoplasmic sperm injection and chromosome analysis of blastocyst after activation. Front Endocrinol (Lausanne) 2021; 12: 692082. doi: 10.3389/fendo.2021.692082.

24. Gebrauchsanweisung_GM508_CultActive_Multi_440X520_55x65_Rev01_00.pdf. 2023 [online]. Available from: https: //gynemed.de/wp-content/uploads/2019/03.

25. Shibahara T, Fukasaku Y, Hayashi N et al. Early rescue oocyte activation for activation-impaired oocytes with no second polar body extrusion after intracytoplasmic sperm injection. J Assist Reprod Genet 2021; 38 (5): 1061–1068. doi: 10.1007/s10815-021-02089-1.