Antioxidant activity of extracts and HPLC analysis of flavonoids from Capella bursa-pastoris (L.) Medik

Česká verzia

Authors: Renata Kubínová; Věra Špačková; Emil Švajdlenka; Katarina Lučivjanská
Authors place of work: Veterinární a farmaceutická univerzita, Farmaceutická fakulta, Ústav přírodních léčiv, Brno
Published in the journal: Čes. slov. Farm., 2013; 62, 174-176
Category: Původní práce

Summary

The flavonoid profile of Capsella bursa-pastoris (L.) Medik. (Brassicaceae) and the antioxidant activity of its methanolic and aqueous extracts were studied. Glycosides of quercetin, chrysoeriol, kaempferol, and isorhamnetin were identified. Chrysoeriol O-glucoside and isorhamnetin O-rutinoside were detected in this species for the first time. The extracts presented an antioxidant activity against DPPH radicals, peroxyl radicals, hydroxyl radicals, and hydrogen peroxide.

Keywords:
Capsella bursa-pastoris • flavonoids • antioxidant activity

Zdroje

1. Grosso C., Vinholes J., Silva L. R., de Pinho P. G., Gonçalves R. F., Valentão P., Jäger A. K., Andrade P. B. Chemical composition and biological screening of Capsellabursa-pastoris. Rev. Bras. Farmacogn. 2011; 21, 635–644.

2. Kweon M. H., Kwak J. H., Ra K. S., Sung H. Ch., Yang H. Ch. Structural characterization of a flavonoid compounds cavenging superoxide anion radica lisolated from Capsellabursa-pastoris. J. Biochem. Mol. Biol. 1996; 29, 423–428.

3. Griffithsa D. W., Deightona N., Bircha A. N. E., Patrianb B., Baurb R., Städler E. Identification of glucosinolates on theleafsurface of plants from the Cruciferae and other closely related species. Phytochemistry 2001; 57, 693–700.

4. Jugea N., Mithena R. F., Trakaa M. Molecular basis for chemoprevention by sulforaphane: a comprehensive review. Cell. Mol. Life Sci. 2007; 64, 1105–1127.

5. Glawischnig E. Camalexin. Phytochemistry 2007; 68, 401–406.

6. Onyilagha J., Bala A., Hallett R., Gruber M., Soroka J., Westcott N. Leaf flavonoids of the cruciferous species, Camelina sativa, Crambespp., Thlaspiarvense and several other genera of the family Brassicaceae. Biochem. Syst. Ecol. 2003; 31, 1309–1322.

7. Blois M. S. Antioxidant determinativ by the use of a stable free radical. Nature 1958; 181, 1199–1200.

8. Ritov B. R., Goldma R., Stoyanovsky A. D., Menshikova E. V., Kagan E. V. Antioxidant paradoxes of phenolic compounds: peroxyl radiál scavenger nad lipid antioxidant, etoposide (VP-16), inhibits sarcoplasmatic reticulum Ca²⁺ -ATPase via thiol oxidation by its phenoxyl radical. Arch. Biochem. Biophys. 1995; 321, 140–152.

9. Parejo I., Petrakis C. H., Kefalas P. J. A transition metal enhanced luminal chemiluminescence in the presence of a chelator. Pharmacol. Toxicol. Method 2000; 43, 183–190.

10. Guo S., Deng Q., Xiao J., Xie B., Sun Z. Evaluation of antioxidant aktivity and preventing DNA damage effect of pomegranate extracts by chemiluminiscence method. J. Agric. Food. Chem. 2007; 55, 3134–3140.

11. Cao G., Sofic E., Prior R. L. Antioxidant and prooxi dant behavior of flavonoids: structure-activity relationships. Free Radic. Biol. Med. 1997; 22, 749–760.