Imunomodulační aktivita etanolových extraktů z Galium verum L. herb.

Imunomodulační aktivita etanolových extraktů z Galium verum L. herb.

V tomto článku jsou diskutovány výsledky výzkumu fytochemického profilu a imunomodulační aktivity etanolických extraktů Galli veri herba (Galium verum L., Rubiaceae). Studované extrakty se získaly z rostlinného materiálu macerací za tepla 20% vodným roztokem ethanolu (tekutý extrakt I), 60% vodným roztokem ethanolu (tekutý extrakt II) a 96% ethanolem (tekutý extrakt III). Obsah hydroxycinnamických derivátů, flavonoidů a polyfenolů byl stanoven spektrofotometricky; polysacharidy byly kvantifikovány gravimetricky; imunomodulační aktivita studovaných látek byla stanovena reakcí blastické transformace lymfocytů (RLBT). Bylo zjištěno, že tekutý extrakt I obsahoval 2,4 % polysacharidů, 3,1 % hydroxycinnamických derivátů, 0,24 % flavonoidů a 2,9% polyfenolů; tekutý extrakt II obsahoval 4,13 % hydroxycinnamových derivátů, 0,16 % flavonoidů a 3,84 % polyfenolů; tekutý extrakt III obsahoval 2,7 % hydroxycinnamických derivátů, 0,18 % flavonoidů a 2,7 % polyfenolů. Všechny studované extrakty vykazovaly výrazný stimulační účinek na transformační aktivitu imunokompetentních krevních buněk. Nejvyšší imunomodulační aktivita byla stanovena pro 96% ethanolový extrakt: procento lymfocytů proliferujících v RLBT vlivem tohoto extraktu se zvýšilo o 6,77–8,04krát ve srovnání s jejich spontánní transformací a 1,14–1,36krát ve srovnání s fytohemaglutininem. Získané výsledky dávají důvody pro další výzkum mechanizmů imunomodulační aktivity extraktů z natě G. verum.

Klíčová slova: ethanolické extrakty • Galium verum L. • imunomodulační aktivita • blastická transformace lymfocytů

Autoři: Igor L. Shinkovenko; Natalia V. Kashpur; Tetiana V. Ilina; Alla M. Kovalyova; Olga V. Goryacha; Oleh M. Koshovyi; Olena V. Kryvoruchko; Andryi M. Komissarenko
Vyšlo v časopise: Čes. slov. Farm., 2018; 67, 101-106
Kategorie: Původní práce

Souhrn

Klíčová slova: ethanolické extrakty • Galium verum L. • imunomodulační aktivita • blastická transformace lymfocytů

Zdroje

1. Aliyev S. C., Chalilov E. N., Veliyeva M. N., Memedova-Ozel H., et al. The implementation of immunotropic and anti-inflammatory activities of “Azeomed” and “Glycyrrhiza glabra” preparations. In: Abstracts of International Symposium on Medicinal Chemistry Research and Development. Kuşadası 2005; 83.

2. Bałan B. J., Różewski F., Zdanowski R., Skopińska-Różewska E. Immunotropic activity of Echinacea. Part I. History and chemical structure. Centr. Eur. J. Immunol. 2012; 37(1), 45–50.

3. Nagarathna P. K. M., Reena K., Sriram R, Wesley J. Review on Immunomodulation and Immunomodulatory Activity of Some Herbal Plants. Int. J. Pharm. Sci. Rev. Res. 2013; 22(1), 223–230.

4. Nfambi J., Bbosa G. S., Sembajwe L. F., Gakunga J., et al. Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in Wistar albino rats. J. Basic Clin. Physiol. Pharmacol. 2015; 26(6), 603–611.

5. Barcz E., Rogala E., Glinkowska G., Strzelecka H., et al. Immunotropic activity of plant extracts. IV. The effect of phenolic compounds of poplar leaves (Populus nigra L.) extract on human leukocytes movement. Herba Pol. 1998; 44, 45–51.

6. Chiang L. C., Ng L. T., Chiang W., Chang M. Y., et al. Immunomodulatory activities of flavonoids, monoterpenoids, triterpenoids, iridoid glycosides and phenolic compounds of Plantago species. Planta Med. 2003; 69 (7), 600–604.

7. Grigore A. Plant Phenolic Compounds as Immunomodulatory Agents, Phenolic Compounds – Biological Activity. https://www.intechopen.com/books/phenolic-compounds-biological-activity/plant-phenolic-compounds-as-immunomodulatory-agents.

8. Lukashov P. I., Moiseev D. V., Stolyarova V. N., Makaremko M. N. Pharmacological activity of caffeic acid. Vestnic Pharmacii 2012; 3(57), 61–65 (in Russian).

9. Dabrowski M. P., Stankiewicz W., Kubacki R., Sobiczewska E., et al. Immunotropic effects in cultured human blood mononuclear cells pre-exposed to low-level 1300 M Hz pulse-modulated microwave field. Electromagnetic Biol. Med. 2003; 22 (1), 1–13.

10. Kashpur N. V., Yurchenko N. S., Ilyina T. V., Kovalyova A. M., et al. The immunomodulatory effect of Asperula odorata L. and Asperula humifusa M. Bieb. Besser dry extracts. Clinical Pharmacy 2015; 19(1), 56–58.

11. Shinkovenko I. L., Kashpur N. V., Ilyina T. V., Kovalyova A. M., et al. The immunomodulatory activity of the extracts and complexes of biologically active compounds of Galium verum herb. Čes. slov. Farm. 2018; 67, 25–29.

12. The State Pharmacopoeia of Ukraine/State enterprise “Scientific and Expert Pharmacopoeial Centre”, First edition, fourth supplement. Kharkiv: RІREG 2011; 538 p. (in Ukrainian).

13. Korulkin D. Y., Abilov D. A., Muzychkina R. A., Tolstikov G. A. Natural flavonoids. Novosibirsk: Geo 2007.

14. Mykchailenko O. O., Kovalyov V. M. Phenolic compounds of the genus Iris plants (Iridaceae). Čes. slov. Farm. 2016; 65, 70–77.

15. The State Pharmacopoeia of Ukraine/State enterprise “Scientific and Expert Pharmacopoeial Centre”, First edition. Kharkiv: RІREG 2001; 556 p. (in Ukrainian).

16. The State Pharmacopoeia of Ukraine/State enterprise “Scientific and Expert Pharmacopoeial Centre”, First edition, first supplement. Kharkiv: RІREG 2004; 494 p. (in Ukrainian).

17. Yezerska O., Kalynyuk T., Vronska L. Quantitative determination of hydroxycinnamic acids in Chicory root. Chemistry and Chemical Technology 2013; 7(3), 247–250.

18. Spagnol C. M., Oliveira Th. S., Lucia Borges V. I., Corrêa M. A., et al. Validation of caffeic acid in emulsion by UV-Spectrophotometric method. Physical Chemistry 2015; 5(1), 16–22.

19. The State Pharmacopoeia of Ukraine/State enterprise “Scientific and Expert Pharmacopoeial Centre”, First edition, second supplement. Kharkiv: RІREG 2008; 617 p. (in Ukrainian).

20. Kovalyova A. A., Georgievskiy G. V., Kovalyov V. M., Komisarenko A. M., et al. Development of new piflamin medicine standardization methods. Farmakom. 2002; 2, 92–97 (in Ukrainian).

21. Korneeva M. N., Novokhatskii A. S., Grebenyuk V. N., Kerimov S. G. Use of the lymphocyte blast transformation reaction to assess the state of cellular immunity. Bulletin of Experimental Biology and Medicine 1989; 107(4), 533–535.

22. Bashirova D. K., Kochnev O. S., Davletkil’deev F. A., Lagutina M. V. Immunologic activity of human lymph cells in the lymphocyte blast transformation reaction. Biull. Eksp. Biol. Med. 1980; 89(1), 33–35.

23. Bulanova E. G., Budagyan V. M., Yarilin A. A., Mazurenko N. N. Expression of Protooncogenes during Lymphocyte Activation by Growth Factors. http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62091191.html

24. Zulfiqar A., Bhaskar S. B. Basic statistical tools in research and data analysis. Indian J. Anaesth. 2016; 60(9), 662–669.

25. Zhao C., Shao J., Cao D., Zhang Y., et al. Chemical constituents of Galium verum. Zhongguo Zhong Yao Za Zhi 2009; 34(21), 2761–2764.

26. Vlase L., Mocan A., Hanganu D., Benedec D., et al. Comparative study of polyphenolic content, antioxidant and antimicrobial activity of four Galium species (Rubiaceae). Digest Journal of Nanomaterials and Biostructures 2014; 9(3), 1085–1094.

27. Ghiţă G., Necula R., Trifan A., Gille E., et al. Investigations regarding the phytochemical study of some samples of Galium verum L. and Galium album Mill. Analele Ştiinţifice ale Universităţii “Al. I. Cuza” Iaşi s. II a. Biologie vegetală 2012; 58(1), 45–50.

28. Tamas M., Stana D., Timis S. Comparative phytochemical research of Galium verum and G. mollugo L. Not. Bot. Hort. Agrobot. Cluj 2006; 34, 18–20.

29. Layali I., Ebrahimzadeh M. A., Joulaei M. Antioxidant properties of Galium verum. International Journal of Life Science and Pharma Research 2016; 6(3), 31–37.

30. Zeng K., Thompson K. E., Yates C. R. Miller D. D. Synthesis and Biological Evaluation of Quinic Acid Derivatives as Anti-Inflammatory Agents. Bioorg. Med. Chem. Lett. 2009; 19, 5458–5460.

31. Amaral S., Mira L., Nogueira J. M., da Silva A. P., et al. Plant extracts with anti-inflammatory properties – a new approach for characterization of their bioactive compounds and establishment of structure-antioxidant activity relationships. Bioorg. Med. Chem. 2009; 17, 1876–1883.

32. Amirghofran Z., Javidnia K., Bahmani M., Azadmehr A., et al. The effect of the methanol extract of Galium mite on the cellular immunity and antibody synthesis. Journal of Immunoassay and Immunochemistry 2011; 32, 157–169.

33. Vlase L., Mocan A., Hanganu D., Benedec D., et al. Comparative study of polyphenolic content, antioxidant and antimicrobial activity of four Galium species (Rubiaceae). Dig. J. Nanomater Biostruct. 2014; 9, 1085–1094.

34. Matei A. O., Gatea F., Dumitra T. E., Radu G. L. Polyphenols analysis from different medicinal plants extracts using capillary zone electrophoresis (CZE)/Revista de Chimie 2016; 67(6), 1051–1055.

35. Raso G. M., Meli R., Di Carlo G., Pacilio M. et al. Inhibition of inducible nitric oxide synthase and cyclooxygenase-2 expression by flavonoids in macrophage J774A.1. Life Sci. 2001; 68(8), 921–931.

36. Sharma M. L., Singh B., Chandan B. K., Khajuria A., et al. Actions of some flavonoids on specific and non-specific immune mechanisms. Phytomedicine 1996; 3(2), 191–195.

37. Sneha J. Anarthe S., Sandhya Rani D., Ganga Raju M. Immunomodulatory activity for methanolic extract of Trigonella foenum graecum whole plant in wistar albino rats. Am. J. Phytomed. Clin. Therapeutics 2014; 2(9), 1081–1092.

38. Tripathi S., Maurya A. K., Kahrana M., Kaul A., et al. Immunomodulatory activity of ethanolic extract of Trigonella foenum graecum leaves on mice. Sch. Res. Lib. 2012; 4(2), 708–713.

39. Sadowska B., Micota B., Różalski M., Redzynia M., et al. The immunomodulatory potential of Leonurus cardiaca extract in relation to endothelial cells and platelets. Innate Immunity 2017; 23(3), 285–295.

40. Gonzalez-Gallego J., Garcıa-Mediavilla M. V., Sanchez-Campos S., Tunon M. J. Fruit polyphenols, immunity and inflammation. Br. J. Nutr. 2010; 104, 15–27.