West Nile virus (lineage 2) in mosquitoes in southern Moravia – awaiting the first autochthonous human cases

Česká verzia

Authors: I. Rudolf ^1,2 ; H. Blažejová ¹; O. Šebesta ^1,3; J. Mendel ¹ ; J. Peško ¹; L. Betášová ¹; P. Straková ¹; S. Šikutová ¹ ; Z. Hubálek ^1,2
Authors place of work: Ústav biologie obratlovců AV ČR, v. v. i., Květná 8, Brno ¹; Ústav experimentální biologie, Masarykova univerzita, Brno ²; Krajská hygienická stanice Jihomoravského kraje se sídlem v Brně ³
Published in the journal: Epidemiol. Mikrobiol. Imunol. 67, 2018, č. 1, s. 44-6
Category: Krátké sdělení

Summary

Here we report repeated detection of lineage 2 West Nile virus (WNV-2) from Culex modestus and Cx. pipiens mosquitoes collected at fishponds in the Lednice-Valtice Area during the mosquito seasons 2015 and 2016. The WNV strains recovered share genetic homology with WNV strains isolated during an extensive monitoring in 2013 as well as with strains circulating in southern and central Europe at the same time. Repeated detection of WNV indicates its establishment in the area and also warns infection specialists and epidemiologists about possible emergence of human cases or even outbreaks of West Nile fever in the region.

Keywords:
West Nile virus – arboviruses – emerging zoonoses –mosquitoes – Culex pipiens – Culex modestus

Zdroje

1. Hubálek Z, Rudolf I. Microbial Zoonoses and Sapronoses. Dordrecht: Springer;2011.

2. Gossner CM, Marrama L, Carson M, et al. West Nile virus surveillance in Europe: moving towards an integrated animal-human-vector approach. Eurosurveill 2017;22:10−19.

3. Hubálek Z, Halouzka J, Juřicová Z. West Nile fever in Czechland. Emerg Infect Dis 1999; 5:594–595.

4. Bakonyi T, Hubálek Z, Rudolf I, et al. Novel Flavivirus or New Lineage of West Nile Virus, Central Europe. Emerg Infect Dis 2005;1:225−231.

5. Hubálek Z, Halouzka J, Juřicová Z, et al. Serologic survey of birds for West Nile flavivirus in southern Moravia (Czech Republic). Vector-borne and Zoonotic Dis 2008;8:659−666.

6. Straková P, Šikutová S, Jedličková P, et al. The Common Coot as sentinel species for the presence of West Nile and Usutu flaviviruses in Central Europe. Res Vet Sci 2015;102:159−161.

7. Sedlák K, Zelená H, Křivda V, et al. Surveillance západonilské horečky u koní v České republice v letech 2011−2013. Epidemiol Mikrobiol Imunol 2014;63:307−311.

8. Rudolf I, Bakonyi T, Šebesta O, et al. West Nile virus lineage 2 isolated from Culex modestus mosquitoes in the Czech Republic, 2013: expansion of the European WNV endemic area to the North? Euro Surveill 2014;19(31):pii=20867.

9. Šebesta O, Gelbič I, Minář J. Mosquitoes (Diptera: Culicidae) of the Lower Dyje River Basin (Podyjí) at the Czech–Austrian border. Cent Eur J Biol 2012;7:288−298.

10. Becker N, Petrič D, Zgomba M, et al. Mosquitoes and their control. Heidelberg: Springer; 2010.

11. Scaramozzino N, Crance JM, Jouan A, et al. Comparison of flavivirus universal primer pairs and development of a rapid, highly sensitive heminested reverse transcription-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequences. J Clin Microbiol 2001;39:1922–1927.

12. Popović N, Milošević B, Urošević A, et al. Outbreak of West Nile virus infection among humans in Serbia, August to October 2012. Euro Surveill 2013;18(43):pii=20613.

13. Vlčková J, Rupeš V, Horáková D, et al. Rizika šíření viru západonilské horečky v České republice. Epidemiol Mikrobiol Imunol 2015;64:80−86.