Comparison of two most frequent methods for the determination of total bilirubin in newborns.

Česká verzia

Authors: T. Šálek ¹; J. Macko ²; P. Šafařík ¹; M. Pšenčík ¹
Authors place of work: Oddělení klinické biochemie, Krajská nemocnice Tomáše Bati, a. s., Havlíčkovo nábřeží 600, 76 75 Zlín, ČR ¹; Oddělení neonatologie, Krajská nemocnice Tomáše Bati, a. s., Havlíčkovo nábřeží 600, 762 75 Zlín, ČR ²
Published in the journal: Klin. Biochem. Metab., 23 (44), 2015, No. 1, p. 17-20

Summary

Aim:
The aim of the study is to compare determination of total bilirubin by direct spectrophotometric method in whole venous blood and by indirect Jendrassik-Gróf method in venous serum in 437 newborns born in Tomas Bata hospital in Zlin.

Design:
retrospective cross section design.

Methods:
Determination of total bilirubin by direct spectrophotometric method in whole venous blood was performed on Radiometer ABL 835 analyzer. Determination of total bilirubin in venous serum by indirect Jendrassik-Gróf was performed on Abbott Architect analyzer. Both methods are metrologically traceable to reference material NIST SRM 916a.

Results:
The median (interquartile range) of serum venous bilirubin was 228 µmol/L (186-263). The median of venous whole blood total bilirubin was 237 µmol/L (192-287). The significant difference between medians was found (p<0,0001). Bland-Altman plot and Passing-Bablok regression show that direct spectrophotometric method of whole venous blood gave higher results than Jendrassik-Gróf test in venous serum mainly in the concentration range above 280 µmol/l (p<0,0001).

Conclusions:
Direct spectrophotometric results of total bilirubin in whole venous blood in the concentration range above 280 umol/l should be confirmed by indirect serum determination in clinical biochemistry laboratory.

Keywords:
bilirubin, neonatal jaundice, hemolysis, interferences.

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