The effect of docetaxel on molecular melting profile of DNA extracted from human breast adenocarcinoma MCF-7 cells

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Aim:
The aim of this study was to evaluate the genotoxic effect of docetaxel on the human adenocarcinoma MCF-7 cells to detect genetic variations and discover potential associations between the drug and the genotoxic damage of MCF-7 cells. Materials and Methods: High resolution melt analysis (HRM) of genomic DNA isolated from MCF-7 cells was used. Two primers of MDR1 gene were selected: 5´-TGGGGCTTTTAGTGTTGGAC-3´, 5´-TGTGGAGAGCTGGATAAAGTCA-3´.

Results:
The significant alterations in the melting temperature Tm of DNA treated with docetaxel in the concentrations of 1 µmol/l and 250 µmol/l were observed. Comparing G+C/A+T ratios the increase of the relative content of G, C was detected. Minor changes of the nucleotide content were observed when compared the sequences of DNA for the control and the docetaxel treated group.

Conclusions:
Using MDR1 primer pairs, our results confirmed that MCF-7 cells are susceptible to genomic DNA instability when exposed to docetaxel.

Keywords:
MCF-7 cell line, high-resolution melting analysis, docetaxel

Autoři: Marianna Trebuňová ¹; Ján Rosocha ¹; Galina Laputková ²; Mario Jančošek ³; Jozef Živčák ⁴
Působiště autorů: Associated Tissue Bank UNLP Košice, Rastislavova 4 , 0 1 90 Košice, Slovakia ¹; Department of Medical and Clinical Biophysics, Faculty of Medicine, P. J. Šafarik University ²; University of Presov in Presov, 17. novembra 15, 080 01 Prešov, Slovakia ³; Faculty of Mechanical Engineering, Department of Biomedical Engineering and Measurement, Technical University, Letná 9, Košice, Slovak Republic ⁴; Trieda SNP 1, Košice, 00 11, Slovakia ⁴
Vyšlo v časopise: Lékař a technika - Clinician and Technology No. 1, 2014, 44, 43-48
Kategorie: Původní práce

Souhrn

Conclusions:
Using MDR1 primer pairs, our results confirmed that MCF-7 cells are susceptible to genomic DNA instability when exposed to docetaxel.

Keywords:
MCF-7 cell line, high-resolution melting analysis, docetaxel

Zdroje

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