Multi-electrode microfluidic platform for protein detection using electrochemical impedance spectroscopy
An impedimetric sensor was developed based on parallel detection at two identical microfluidic channels with 3 pairs of interdigitated electrodes in each. Preparation and cleaning of the gold electrodes were examined and optimized. A self-assembling monolayer of 11-mercaptopropionic acid was deposited to form a base for later covalent binding of antibody proteins via EDC/NHS chemistry. Two immunoassays containing immune reaction of a 214D4 antibody and a corresponding MUC1ex antigen and a prostate antigen antibody immunoassay with positive and negative controls were performed in two parallel microfluidic channels. Presented platform enables a simultaneous analysis of the protein reactions on multiple electrodes leading to an improvement in on-time diagnostics and point-of-care.
Keywords:
Impedance sensor, protein adhesion, antigen – antibody interaction
Autoři:
Jaroslav Lazar 1; Magdalena Bialon 2; Christiane Püttmann 2; Christoph Stein 2; Wilfred Germeraad 3; Uwe Schnakenberg 1
Působiště autorů:
Institute of Materials in Electrical Engineering 1, RWTH Aachen University, Sommerfeldstr. 4, Aachen, Germany
1; Helmholtz Institute for Biomedical Engineering, Institute of Applied Medical Engineering, Dept. of Experimental Medicine and Immunotherapy, RWTH Aachen University, Pauwelsstr. 20, 52074 Aachen, Germany
2; Department of Immunology, Maastricht University Medical Center, Medical Center, Universiteitssingel 50, 6229 Maastricht, the Netherlands
3
Vyšlo v časopise:
Lékař a technika - Clinician and Technology No. 4, 2015, 45, 105-109
Kategorie:
Původní práce
Souhrn
An impedimetric sensor was developed based on parallel detection at two identical microfluidic channels with 3 pairs of interdigitated electrodes in each. Preparation and cleaning of the gold electrodes were examined and optimized. A self-assembling monolayer of 11-mercaptopropionic acid was deposited to form a base for later covalent binding of antibody proteins via EDC/NHS chemistry. Two immunoassays containing immune reaction of a 214D4 antibody and a corresponding MUC1ex antigen and a prostate antigen antibody immunoassay with positive and negative controls were performed in two parallel microfluidic channels. Presented platform enables a simultaneous analysis of the protein reactions on multiple electrodes leading to an improvement in on-time diagnostics and point-of-care.
Keywords:
Impedance sensor, protein adhesion, antigen – antibody interaction
Zdroje
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[10] PUETTMANN, C., KOLBERG, K., HAGEN, S., SCHMIES, S., FISCHER, R., NAEHRING, J., BARTH, S., A monoclonal antibody for the detection of SNAP/CLIP-tagged proteins. Immunology letters, 2012, PMID 23085606.
[11] VAN ELSSEN, C. H., FRINGS, P. W., BOT, F. J., VAN DE VIJVER, K. K., HULS, M. B., MEEK, B., HUPPERETS, P., GERMERAAD, W. T., BOS, G.M. Expression of aberrantly glycosylated Mucin-1 in ovarian cancer. Histopathology, 2010, 57/4, p. 597–606.
[12] ZOU, Z., et al. Functionalized nano interdigitated electrodes arrays on polymer with integrated microfluidics for direct bio-affinity sensing using impedimetric measurement. Sensors and Actuators A: Physical, 2007, Vol. 136, Nr. 2, p. 518–526.
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Štítky
BiomedicínaČlánok vyšiel v časopise
Lékař a technika
2015 Číslo 4
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