One-Step Nucleic Acid Amplification method – what is the future of sentinel lymph node management?

Česká verzia

Authors: J. Kosťun ¹; M. Pešta ²; R. Slunečko ⁴; P. Vlasák ¹; J. Bouda ¹; D. Berezovskiy ¹; O. Topolčan ³; Z. Novotný ¹; Jiří Presl ¹
Authors place of work: Gynekologicko-porodnická klinika FN a LF UK, Plzeň, přednosta doc. MUDr. Z. Novotný, CSc. ¹; Ústav biologie LF UK, Plzeň, vedoucí doc. RNDr. M. Pešta, Ph. D. ²; Oddělení imunochemické diagnostiky FN a LF UK, Plzeň, primář prof. MUDr. O. Topolčan, CSc. ³; Šiklův ústav patologie FN a LF UK, Plzeň, přednosta prof. MUDr. M. Michal ⁴
Published in the journal: Ceska Gynekol 2018; 83(1): 57-61

Summary

Objective:
The article summarizes current possibilities of usage of the One-Step Nucleic Acid Amplification method (OSNA) in the perioperative management of sentinel lymph nodes in oncologic surgery. The principle of this method is the detection of cytokeratin 19 (CK19) in the lymphatic tissue as a marker of the metastatic spread.

Design:
Review article.

Settings:
Department of Obstetrics and Gynaecology, University Hospital Pilsen, Faculty of Medicine in Pilsen, Charles University, Prague; Department of Biology, Faculty of Medicine in Pilsen, Charles University, Prague; Department of Immunochemistry, University Hospital Pilsen, Faculty of Medicine in Pilsen, Charles University, Prague; Sikl´s Department of Pathology, University Hospital Pilsen, Faculty of Medicine in Pilsen, Charles University, Prague.

Methods:
The review of the literature published until the end of April 2017 available on the PubMed database was performed. The official abbreviation OSNA and the full name of the method One-Step Nucleic Acid Amplification was used for search in this database.

Conclusion:
The usage of the OSNA method with the detection of CK 19 in the sentinel lymph nodes as a marker of metastatic spread to the lymphatic tissue currently represents an acceptable form of perioperative sentinel lymph node management in patients with breast and colorectal cancer. Until now published data are pointing towards possible successful application of this method in sentinel lymph node management in patients with some other malignancies, such as thyroid carcinoma, gastric cancer, uterus cancer and head and neck cancer. More data is needed to establish this method also in those neoplasms.

Keywords:
OSNA, CK19, sentinel lymph node

Zdroje

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