Impact of modification of implants for replacement of osteochondral defects on the gene expression of chondrocytes
Authors:
M. Polanská 1; H. Hulejová 1; M. Petrtýl 2; Z. Bastl 3; Z. Kruliš 4; Z. Horák 4; D. Veigl 5; L. Šenolt 1
Authors place of work:
Revmatologický ústav, Praha
1; České vysoké učení technické, Fakulta stavební, Praha
2; Ústav fyzikální chemie, Akademie věd, Praha
3; Ústav molekulární chemie, Akademie věd, Praha
4; I. ortopedická klinika 1. LF UK a FN Motol, Praha
5
Published in the journal:
Čes. Revmatol., 17, 2009, No. 1, p. 17-22.
Category:
Original Papers
Summary
Introduction:
Polymers represent materials suitable for replacement of subchondral defects. The aim of this study was to evaluate the impact of superficial modification of cycloolefin copolymer (COC) and COC blend with collagen type II on the viability and gene expression of chondrocytes.
Material and methods:
Human chondrocytes were grown in cell culture medium on the surface of COC and COC blend biomaterials. The surface of a half of the materials was plasmatically modified by atoms of nitrogen and oxygen for bond of collagen type II. The gene expression of matrix metalloproteinases (MMP-1,-3,-13), proinflammatory cytokines (IL-1, TNF-alfa) and apoptotic molecules (BAX, Bcl-2) was evaluated by quantitative Taq-Man PCR after 48 hours of incubation. Viability of chondrocytes was evaluated by MTT test after 2, 4, and 8 days of incubation. The synthesis of MMPs was measured by ELISA in cell medium after 48 hours of incubation.
Results:
Chondrocytes cultured on the surface of copolymers plasmatically modified had an average mRNA expression 2.8-fold increased for IL-1 and 8.2-fold increased for MMP-1. All of tested MMPs were increasingly produced into cell medium by chondrocytes cultured on the plasmatically modified surface of expression of MMPs mRNA. Modified materials, compared to unmodified polymers, decreased viability of chondrocytes according to the length of exposition. The gene expression of TNF-α and apoptotic molecules by chondrocytes did not differ among tested materials.
Conclusion:
Cycloolefin copolymers COC and COC blend can represent suitable materials for tissue engineering, but their plasmatic modification can, at least in „in vitro“ conditions, decrease viability of chondrocytes and induce their pro-destructive potential. The advantages and disadvantages of the plasmatic modification of materials for replacement of osteochondral defects may be unveiled by further studies.
Key words:
osteochondral defects biomaterials, chondrocytes, gene expression, cytokines
Zdroje
1. Goldring MB, Goldring SR. Osteoarthritis. J Cell Physiol 2007; 213: 626–34.
3. van der Kraan PM, Buma P, van Kuppevelt T, van den Berg WB. Interaction of chondrocytes, extracellular matrix and growth factors: relevance for articular cartilage tissue engineering. Osteoarthritis Cartilage 2002; 10: 631–7.
4. Hedbom E, Häuselmann HJ. Molecular aspects of pathogenesis in osteoarthritis: the role of inflammation. Cell Mol Life Sci 2002; 59: 45–53.
5. Del Carlo M. Jr, Loeser RF. Cell death in osteoarthritis. Curr Rheumatol Rep 2008; 10: 37–42.
6. Chajra H, Rousseau CF, Cortial D, RonziŹre MC, Herbage D, Mallein-Gerin F, Freyria AM. Collagen-based biomaterials and cartilage engineering. Application to osteochondral defects. Biomed Mater Eng 2008;18: S33–45.
7. Martin I, Miot S, Barbero A, Jakob M, Wendt D. Osteochondral tissue engineering. J Biomech 2007; 40: 750–65.
8. Hulejová H, Adam M, Petrtýl M. Cycloolefin Copolymers. Bone. 2005; 36: 172–173.
9. Zwingmann J, Mehlhorn AT, Südkamp N, Stark B, Dauner M, Schmal H. Chondrogenic differentiation of human articular chondrocytes differs in biodegradable PGA/PLA scaffolds. Tissue Eng 2007; 13: 335–43.
10. Ramakrishna S., Mayer J, Wintermantel E, Leong KW. Biomedical applications of polymer-composite materials: a review. Composites Science and Technology 2001; 61: 1189–1224.
11. Cornelis M, Dupont C, Wepierre J. In vitro cytotoxicity tests on cultured human skin fibroblasts to predict the toxic potential of surfactants. ATLA.1991; 19: 324–337.
12. Tanzi MC, Verdeiro P, Lampugnani MG, Resnati M, Dejana E, Sturani E. Cytotoxicity of some catalysts commonlyused in the synthesis of copolymers for biomedical use. J. Mat. Sci.: Mat. Med. 1992; 10: 9–12.
13. Willumeit R, Schossig M, Clemens H, Feyerabend F. In-vitro interactions of human chondrocytes and mesenchymal stem cells, and of mouse macrophages with phospholipid-covered metallic implant materials. Eur Cell Mater 2007; 13: 11–25.
14. Shaw AJ. Defining cell viability and cytotoxicity. ATLA. 1994; 22: 124–126.
15. Pérez Olmedilla M, Garcia-Giralt N, Pradas MM, et al. Response of human chondrocytes to a non-uniform distribution of hydrophilic domains on poly (ethyl acrylate-co-hydroxyethyl methacrylate) copolymers. Biomaterials 2006; 27: 1003–12.
16. Chajra H, Rousseau CF, Cortial D, RonziŹre MC, Herbage D, Mallein-Gerin F, Freyria AM. Collagen-based biomaterials and cartilage engineering. Application to osteochondral defects. Biomed Mater Eng 2008; 18: 33–45.
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