Immunophenotyping and other applications of flow cytometry in acute leukemias
Authors:
O. Hrušák; E. Mejstříková; V. Kanderová; M. Vášková; T. Kalina
Authors place of work:
Klinika dětské hematologie a onkologie 2. LF UK a FN Motol, Praha, přednosta prof. MUDr. Jan Starý, DrSc.
Published in the journal:
Čes-slov Pediat 2015; 70 (2): 85-91.
Category:
Acute leukemias in children
Summary
Cytometric immunophenotyping is one of the most powerful methods in acute leukemia diagnostics. Main advantages are its speed, reliability and knowledge of parameters investigated at a single-cell level. Disadvantages may include relative expensiveness compared to some basic methods. Cytometric immunophenotyping should be interpreted by an expert and reported to the clinical physician so that all relevant questions regarding the patient's classification and treatment are addressed adequately. Flow cytometry can evaluate other parameters such as the DNA contents or proteome analysis. Practical diagnostic usefulness of the newest generation multiparametric instruments is being assessed.
Key words:
acute leukemias, children, immunophenotyping, cytometry
Zdroje
1. Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia 2012; 26: 1986–2010.
2. Hrusák O, Porwit-MacDonald A, Hrusak O. Antigen expression patterns reflecting genotype of acute leukemias. Leukemia 2002; 16: 1233–1258.
3. Mejstríková E, Fronková E, Kalina T, et al. Detection of residual B precursor lymphoblastic leukemia by uniform gating flow cytometry. Pediatr Blood Cancer 2010; 54: 62–70.
4. San Miguel JF, Ciudad J, Vidriales MB, et al. Immunophenotypical detection of minimal residual disease in acute leukemia. Crit Rev Oncol Hematol 1999; 32: 175–185.
5. Gaipa G, Basso G, Maglia O, et al. Drug-induced immunophenotypic modulation in childhood ALL: implications for minimal residual disease detection. Leukemia 2005; 19: 49–56.
6. Mejstríková E, Kalina T, Trka J, et al. Correlation of CD33 with poorer prognosis in childhood ALL implicates a potential of anti-CD33 frontline therapy. Leuk J Leuk Soc Am Leuk Res Fund U.K. 2005; 19: 1092–1094.
7. Vaskova M, Mejstrikova E, Kalina T, et al. Transfer of genomics information to flow cytometry: expression of CD27 and CD44 discriminates subtypes of acute lymphoblastic leukemia. Leukemia 2005; 19: 876–878.
8. Vaskova M, Fronkova E, Starkova J, et al. CD44 and CD27 delineate B-precursor stages with different recombination status and with an uneven distribution in nonmalignant and malignant hematopoiesis. Tissue Antigens 2008; 71: 57–66.
9. Zaliova M, Meyer C, Cario G, et al. TEL/AML1-positive patients lacking TEL exon 5 resemble canonical TEL/AML1 cases. Pediatr Blood Cancer 2011; 56: 217–225.
10. Mejstříková E, Pelková V, Kalina T, Hrušák O. Průtoková cytometrie – úloha imunofenotypizace v hematologii a navazujících oborech medicíny. In: Pospíšilová Š, Dvořáková D, Mayer J (eds). Molekulární hematologie. Praha: Galén, 2013.
11. Campo E, Swerdlow SH, Harris NL, et al. The 2008 WHO classification of lymphoid neoplasms and beyond: evolving concepts and practical applications. Blood 2011; 117: 5019–5032.
12. Jaffe ES, Harris NL, Diebold J, Muller-Hermelink HK. World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues. A progress report. Am J Clin Pathol 1999; 111: S8–S12.
13. Hrušák O, Basso G, Ratei R, et al. Flow diagnostics essential (FDE) code: A simple and brief format for the summary of leukemia phenotyping. Cytom Part B Clin Cytom 2013.
14. Bene MC, Castoldi G, Knapp W, et al. Classification of acute leukemias: reply from EGIL to Dr van Dongen. Leukemia 1996; 10: 1363–1364.
15. Bene MC, Castoldi G, Knapp W, et al. Proposals for the immunological classification of acute leukemias. European Group for the Immunological Characterization of Leukemias (EGIL). Leukemia 1995; 9: 1783–1786.
16. Coustan-Smith E, Mullighan CG, Onciu M, et al. Early T-cell precursor leukaemia: a subtype of very high-risk acute lymphoblastic leukaemia. Lancet Oncol 2009; 10: 147–156.
17. Matutes E, Pickl WF, Van’t Veer M, et al. Mixed-phenotype acute leukemia: clinical and laboratory features and outcome in 100 patients defined according to the WHO 2008 classification. Blood 2011; 117: 3163–3171.
18. Bene MC, Bernier M, Casasnovas RO, et al. The reliability and specificity of c-kit for the diagnosis of acute myeloid leukemias and un-differentiated leukemias. The European Group for the Immunological Classification of Leukemias (EGIL). Blood 1998; 92: 596–599.
19. Fujisaki H, Hara J, Takai K, et al. Lineage switch in childhood leukemia with monosomy 7 and reverse of lineage switch in severe combined immunodeficient mice. Exp Hematol 1999; 27: 826–833.
20. Slamova L, Starkova J, Fronkova E, et al. CD2-positive B-cell precursor acute lymphoblastic leukemia with an early switch to the monocytic lineage. Leukemia 2014; 28: 609–620.
21. Mullighan CG. New strategies in acute lymphoblastic leukemia: Translating advances in genomics into clinical practice. Clin Cancer Res 2010; 17: 396–400.
22. Kornblau SM, Tibes R, Qiu YH, et al. Functional proteomic profiling of AML predicts response and survival. Blood 2009; 113: 154–164.
23. Tian Q, Stepaniants SB, Mao M, et al. Integrated genomic and proteomic analyses of gene expression in mammalian cells. Mol Cell Proteomics 2004; 3: 960–969.
24. Perez OD, Nolan GP. Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry. Nat Biotechnol 2002; 20: 155–162.
25. Roberts KG, Morin RD, Zhang J, et al. Genetic alterations activating kinase and cytokine receptor signaling in high-risk acute lymphoblastic leukemia. Cancer Cell 2013; 22: 153–166.
26. Wu W, Slåstad H, de la Rosa Carrillo D, et al. Antibody array analysis with label-based detection and resolution of protein size. Mol Cell Proteomics 2009; 8: 245–257.
27. Stuchly J, Kanderova V, Fiser K, et al. An automated analysis of highly complex flow cytometry-based proteomic data. Cytom A 2012; 81: 120–129.
Štítky
Neonatology Paediatrics General practitioner for children and adolescentsČlánok vyšiel v časopise
Czech-Slovak Pediatrics
2015 Číslo 2
- What Effect Can Be Expected from Limosilactobacillus reuteri in Mucositis and Peri-Implantitis?
- The Importance of Limosilactobacillus reuteri in Administration to Diabetics with Gingivitis
Najčítanejšie v tomto čísle
- Acute myeloid leukemia in children
- Acute lymphoblastic leukemia
- Leukemias in children in the 21st century
- Immunophenotyping and other applications of flow cytometry in acute leukemias