Merkel Cell Carcinoma – Immunohistochemical Study in a Group of 11 Patients
Authors:
T. Jirásek 1; R. Matěj 1,2; L. Pock 3; I. Knotková 1; V. Mandys 1
Authors place of work:
Ústav patologie 3. LF UK a FNKV, Praha
1; Oddělení patologie a molekulární medicíny, FTNsP, Praha
2; Dermatohistopatologická laboratoř, Praha
3
Published in the journal:
Čes.-slov. Patol., 45, 2009, No. 1, p. 9-13
Category:
Original Article
Summary
The aim of our work was to confirm an immunohistochemical profile of routine markers of epithelial and neuroendocrine differentiation in eleven cases of Merkel cell carcinoma, as well as to study the expression of two markers of early phases of neuronal differentiation, namely reelin and class III β-tubulin, markers which have not yet been studied in Merkel cell carcinomas. In all the investigated tumours the characteristic “dot-like” pattern of cytokeratin 20 immunoexpression, as well as negative immunostaining for cytokeratin 7 and thyroid transcription factor 1 (TTF-1) were disclosed; all the tumours showed neuroendocrine differentiation, expressing either neuron specific enolase (NSE) or chromogranin A(CgA), or both. An interesting finding was observed when the anti-cytokeratin monoclonal antibody MNF 116 was used. The characteristic “dot-like” pattern was detected in high proportion of tumours, including two samples of local recurrence of one of the carcinomas, where neoplastic cells have lost the expression of cytokeratin 20. The majority (91%) of Merkel cell carcinomas included in our group showed positive immunodetection of class III β-tubulin when TU-20 antibody was used, while TuJ-1 immunostaining was surprisingly negative in all the investigated tumours. Detection of reelin was negative in almost all the studied Merkel cell carcinomas except for cases, where neoplastic cells revealed weak focal immunostaining in a minor portion of neoplastic cells.
Key words:
Merkel cell carcinoma – class III β-tubulin – Tu-20 – TuJ-1 – reelin
Zdroje
1. Bayrou, O., Avril, M. F., Charpentier, P., et al.: Primary neuroendocrine carcinoma of the skin. Clinicopathologic study of 18
cases. J Am Acad Dermatol., 24, 1991, s. 198–207.
2. Dráberová, E., Lukás, Z., Ivanyi, D., et al.: Expression of class III beta-tubulin in normal and neoplastic human tissues. Histochem
Cell Biol., 109, 1998, s. 231–239.
3. Easter, S. S. Jr., Ross, L. S., Frankfurter, A.: Initial tract formation in the mouse brain. J Neurosci., 13, 1993, s. 285–299.
4. Gaudin, P. B., Rosai, J.: Florid vascular proliferation associated with neural and neuroendocrine neoplasms. A diagnostic clue
and potential pitfall. Am J Surg Pathol., 19, 1995, s. 642–652.
5. Herz, J., Chen, Y.: Reelin, lipoprotein receptors and synaptic plasticity. Nat Rev Neurosci., 7, 2006, s. 850–859.
6. Jensen, K., Kohler, S., Rouse, R. V.: Cytokeratin staining in Merkel cell carcinoma: an immunohistochemical study of cytokeratins
5/6, 7, 17, and 20. Appl Immunohistochem Mol Morphol., 8, 2000, s. 310–315.
7. Jirásek, T., Cipro, S., Musilová, A., et al.: Expression of class III β-tubulin in colorectal carcinomas: an immunohistochemical study
using TU-20 and TuJ-1 antibody. Indian J Med Res. v tisku 8. Jirásek, T., Mandys, V., Viklický, V.: Expression of class III betatubulin
in neuroendocrine tumours of gastrointestinal tract. Folia Histochem Cytobiol., 40, 2002, s. 305–310.
9. Jirásek, T., Písaríková, E., Viklický, V., Mandys, V.: Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies. Folia Histochem Cytobiol., 45, 2007, s. 41–45.
10. Katsetos, C. D., Frankfurter, A., Christakos, S., et al.: Differential localization of class III, beta-tubulin isotype and calbindin-
D28k defines distinct neuronal types in the developing human cerebellar cortex. J Neuropathol Exp Neurol., 52, 1993, s. 655–666.
11. Katsetos, C. D., Kontogeorgos, G., Geddes, J. F., et al.: Differential distribution of the neuron-associated class III beta-tubulin
in neuroendocrine lung tumors. Arch Pathol Lab Med., 124, 2000, s. 535–544.
12. Katsetos, C. D., Legido, A., Perentes, E., Mörk, S. J.: Class III beta-tubulin isotype: a key cytoskeletal protein at the crossroads
of developmental neurobiology and tumor neuropathology. J Child Neurol., 18, 2003, s. 851–866.
13. Kohler, S., Kerl, H.: Merkel cell carcinoma. In: LeBoit, P. E., Burg, G., Weedon, D., et al., eds. World Health Organization
Classification of Tumours. Pathology and Genetics of Skin Tumours. Lyon: IARC Press, 2006, s. 272–273.
14. Kukharskyy, V., Sulimenko, V., Macůrek, L., et al.: Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src
and Fyn in differentiating P19 embryonal carcinoma cells. Exp Cell Res., 298, 2004, s. 218–228.
15. Lobert, S., Frankfurter, A., Correia, J. J.: Binding of vinblastine to phosphocellulose-purified and alpha beta-class III tubulin: the
role of nucleotides and beta-tubulin isotypes. Biochemistry, 34,1995, s. 8050–8060.
16. LudueĖa, R. F.: Are tubulin isotypes functionally significant. Mol Biol Cell., 4, 1993, s. 445–457.
17. Perrone, G., Vincenzi, B., Zagami, M., et al.: Reelin expression in human prostate cancer: a marker of tumor aggressiveness
based on correlation with grade. Mod Pathol., 20, 2007, s. 344–351.
18. Pilotti, S., Rilke, F., Lombardi, L.: Neuroendocrine (Merkel cell) carcinoma of the skin. Am J Surg Pathol., 6, 1982, s. 243–254.
19. Ratner, D., Nelson, B. R., Brown, M. D., Johnson, T. M.: Merkel cell carcinoma. J Am Acad Dermatol., 29, 1993, s. 143–156.
20. Santa Cruz, D. J.: Tumors of the skin. In: Fletcher, C. D. M., ed. Diagnostic Histopathology of Tumors. 3rd ed., Philadelphia:
Churchill Livingstone Elsevier, 2007, s. 1465–1466.
21. Sato, N., Fukushima, N., Chang, R., et al.: Differential and epigenetic gene expression profiling identifies frequent disruption of
the RELN pathway in pancreatic cancers. Gastroenterology, 130, 2006, s. 548–565.
22. Schmid, C., Beham, A., Feichtinger, J., et al.: Recurrent and subsequently metastasizing Merkel cell carcinoma in a 7-year-old
girl. Histopathology, 20, 1992, s. 437–439.
23. Scott, C. A., Walker, C. C., Neal, D. A., et al.: Beta-tubulin epitope expression in normal and malignant epithelial cells. Arch
Otolaryngol Head Neck Surg., 116, 1990, s. 583–589.
24. Wang, Q., Lu, J., Yang, C., et al.: CASK and its target gene Reelin were co-upregulated in human esophageal carcinoma. Cancer
Lett., 179, 2002, s. 71–77.
25. Walsh, N. M.: Primary neuroendocrine (Merkel cell) carcinoma of the skin: morphologic diversity and implications thereof. Hum Pathol., 32, 2001, s. 680–689.
Štítky
Anatomical pathology Forensic medical examiner ToxicologyČlánok vyšiel v časopise
Czecho-Slovak Pathology
2009 Číslo 1
Najčítanejšie v tomto čísle
- Histological Differential Diagnosis of Hydatidiform Moles and Hydropic Abortions
- Post-Radiation Dedifferentiation of Meningioma into Chondroblastic Osteosarcoma
- Merkel Cell Carcinoma – Immunohistochemical Study in a Group of 11 Patients
- Diagnostic Possibility of Celiac Disease in Bioptic Practise