Molecular detection of infectious agents in the pathology laboratory
Authors:
Kateřina Černá 1,2; Radek Šíma 1,2; Jana Němcová 1,2,3
Authors‘ workplace:
Šiklův ústav patologie LF UK a FN Plzeň 2 Bioptická laboratoř s. r. o., Plzeň 3 Biomedicínské centrum Lékařské fakulty v Plzni
Published in:
Čes.-slov. Patol., 60, 2024, No. 4, p. 169-175
Category:
Reviews Article
Overview
Using molecular methods, infectious organisms of viral, bacterial and fungal origin, as well as protozoa and helminths, can be detected. Molecular methods detect specific segments in the nucleic acid sequences of infectious agents and therefore do not require the maintenance of viability of the microorganisms of interest. Therefore, these methods can also be used for direct detection of infectious agents from fixed tissue, the most commonly available material in pathology. This short review article is based on more than 20 years of molecular microbiology within pathology and our aim is to present the possibilities of molecular detection of infectious organisms for pathological diagnosis.
Keywords:
pathology – Microbiome – molecular methods – PCR – infectious agents – FFPE
Sources
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Labels
Anatomical pathology Forensic medical examiner ToxicologyArticle was published in
Czecho-Slovak Pathology
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