Sporicidal Effect of Presept and Chloramine B on Bacillus cereus Spores

České info

Sporicidní působení Preseptu a Chloraminu B na spory Bacillus cereus

Bylo porovnáváno sporicidní působení Preseptu a Chloraminu B o stejné koncentraci aktivního chloru na spory Bacillus cereus. Zatímco část spor po 4hodinovém působení Chloraminu B vyklíčila a počala se v bohatém živném médiu množit, optická hustota kultury naočkované sporami opraco- vanými Preseptem se po 7 hodin nezměnila. Byly zjištěny i významné morfologické rozdíly mezi oběma populacemi spor. Spory opracované Preseptem sice ztratily během 3 hodin v živném médiu impermeabilitu, což se projevilo ztrátou jejich světlolomnosti, ale naprostá většina z nich se už dále nevyvíjela. Část spor opracovaných Chloraminem B se už však během 7 hodin přeměnila na vegetativní buňky. Zdá se proto, že Presept neblokuje ztrátu impermeability spory a její vyklíčení, ale inhibuje její další postgerminativní vývoj a následné buněčné dělení.

Klíčová slova:
sporicidní působení – Presept – Chloramin B – dezinfekce – klíčení spor – Bacillus cereus.

Authors: O. Hlaváček; V. Vinter; J. Chaloupka
Authors‘ workplace: Mikrobiologický ústav, Akademie věd ČR, Praha
Published in: Epidemiol. Mikrobiol. Imunol. , 1998, č. 4, s. 150-153
Category:

Overview

The sporicidal effect of Presept was compared with Chloramine B on the spores of Bacillus cereus. Either compound was calibrated to the same concentration of active chlorine. While a portion of spore population after 4 hrs of treatment by Chloramine germinated and started to divide in a rich nutrient medium, the optical density of the culture inoculated with spores treated by Presept did not increase even after 7 hrs when exposed to the nutrient medium. Significant morphological differences were found in either population of spores. Spores treated by Presept lost the imperme- ability within 3 hrs in the nutrient medium but almost no postgerminative development was observed. However, a portion of spores treated by Chloramine B developed after germination within 3 hrs into vegetative cells. It seems that Presept does not block germination and/or loss of imperme- ability of spores, but prevents their postgerminative development and division.

Key words:
sporicidal effect – Presept – Chloramine B – disinfection – germination of spores – Bacillus cereus.

Full text is not available online.

If interested in a scan of this journal, contact NTO ČLS JEP.

Labels

Hygiene and epidemiology Medical virology Clinical microbiology

Factors Influencing Carriership of Neisseria meningitidis and Neisseria lactamica

Candida krusei (Cast.) Berkhout – a Potential Pathogen of Increasing Importance

Problem of Specifi- city of Serological Diagnostic Tests in Legionellosis

Long-term Investigation of Neisseria meningitidis Carriership in the Nasopharynx and Seroprevalence of Specific Antibodies in Immunized and Non-immunized Sub- jects

Classification of Microscopic Fungi with Regard to Risk of Infection of Laboratory Workers

Concurrent Incidence of Antibodies against Toxoplas- ma gondii and Borrelia burgdorferi in Patients with Suspected Toxoplasmosis or Borreliosis

Salmonellosis Epidemic in the First League Hockey Team

Article was published in

Epidemiology, Microbiology, Immunology

1998 Issue 4