The Use of Molecular Genetics Techniques in Clinical Microbiology – Final Report from the Workshop of the Molecular Microbiology Working Group TIDE

Česká verzia

Authors: J. Hrabák ¹; M. Bunček ²; M. Dendis ³; R. Horváth ^3,9; A. Chroňáková ⁴; A. Libra ²; J. Nešvera ⁵; R. Pantůček ⁶; N. Piskunová ⁷; L. Plíšková ⁸; F. Růžička ⁹; P. Sauer ¹⁰; I. Sedláček ⁶; P. Trubač ⁷; E. Žampachová ⁷; H. Žemličková ¹¹; J. Scharfen ¹²
Authors place of work: Ústav mikrobiologie, Lékařská fakulta UK a Fakultní nemocnice v Plzni, Plzeň ¹; Generi Biotech, Hradec Králové ²; GeneProof, a. s., Brno ³; Ústav půdní biologie, Biologické centrum ČAV, České Budějovice ⁴; Mikrobiologický ústav ČAV, Praha ⁵; Ústav experimentální biologie, Přírodovědecká fakulta, Masarykova univerzita, Brno ⁶; Nemocnice České Budějovice, České Budějovice ⁷; Lékařská fakulta UK a Fakultní nemocnice v Hradci Králové, Hradec Králové ⁸; Mikrobiologický ústav FN u sv. Anny a LF MU, Brno ⁹; Ústav mikrobiologie, Lékařská fakulta UP a Fakultní nemocnice v Olomouci, Olomouc ¹⁰; Národní referenční laboratoř pro antibiotika, Státní zdravotní ústav v Praze, Praha ¹¹; Národní referenční laboratoř pro patogenní aktinomycety, Nemocnice Trutnov, Trutnov ¹²
Published in the journal: Epidemiol. Mikrobiol. Imunol. 59, 2010, č. 3, s. 103-106

Summary

In the last decade, there has been a rapid development in the use of molecular genetics methods in clinical microbiology. Novel technologies bring new knowledge and approaches to various disciplines of microbiology – taxonomy, identification of microbes, clinical diagnosis, epidemiology of infectious diseases and antibiotic resistance. This article summarizes the conclusions from the workshop of the Molecular Microbiology Working Group TIDE held during the Second Annual Meeting of the Society for Medical Microbiology of the J. E. Purkyne Czech Medical Association.

Key words:
molecular microbiology – typing – identification – taxonomy – PCR – clinical microbiology.

Zdroje

1. Vandamme, P., Pot, B., Gillis, M., De Vos, P. et al. Polyphasic taxonomy, a konsensus approach to bacterial systematics. Microbiol. Rev., 1996, 60, p. 407–438.

2. Van Belkum, A., Tassios, P. T., Dijkshoorn, L., Haeggman, S. et al. Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin. Microbiol. Infect., 2007, 13, Suppl. 3, p. 1–46.

3. Čekanová, L., Kolář, M., Chromá, M., Sauer, P. et al. Prevalence of ESBL-positive bacteria in the community in the Czech Republic. Medical Science Monitor, 2009, 15, p. 202–206.

4. Grundmann, H., Aanensen, D. M., van den Vijngaard, C. C., Spratt, B. G. et al. Geographic distribution of Staphylococcus aureus causing invasive infections in Europe: a molecular-epidemiological analysis. PloS Med., 2010, 7, 1, p. e100215.

5. Hrabák, J., Empel, J., Gniadkowski, M., Halbhuber, Z. et al. CTX-M-15-producing Shigella sonnei from a Czech patient who traveled in Asia. Journal of Clinical Microbiology, 2008, 46, p. 2147–2148.

6. Hrabák, J., Empel, J., Bergerová, T., Fajfrlík, K. et al. International clones of Klebsiella pneumoniae and Escherichia coli with extended-spectrum -lactamases (ESBLs) in a Czech hospital. Journal of Clinical Microbiology, 2009, 47, p. 3353–3357.

7. Kolář, M., Sauer, P., Faber, E., Kohoutová, J. et al. Prevalence and spread of Pseudomonas aeruginosa and Klebsiella pneumoniae strains in patients with hematological malignancies. New Microbiologica, 2009, 32, p. 67–76.

8. Mariapia, V. M. (Ed.). Detection of Bacteria, Viruses, Parasites and Fungi. Springer, 2010.

9. Zemlickova, H., Urbaskova, P., Jakubu, V., Motlova, J. et al. Clonal distribution of invasive pneumococci, Czech Republic 1996–2003. Emerg. Infect. Dis., 2010, 16, p. 287–289.

Štítky

Hygiene and epidemiology Medical virology Clinical microbiology

Článek Examination of Mosquitoes Collected in Southern Moravia in 2006–2008 Tested for Arboviruses

Článek Tick-Borne Encephalitis in the East Bohemia Region and its Microbiological Diagnostic Pitfalls

Článek Lipophilic Yeasts of the Genus Malassezia and Skin Diseases. I. Seborrhoeic Dermatitis

Článek Pernicious Anaemia – Diagnostic Benefit of the Detection of Autoantibodies against Intrinsic Factor and Gastric Parietal Cells Antigen H+/K+ ATPase

Článek Prevalence of Anti-Epstein-Barr Virus Antibodies in Children and Adolescents with Secondary Immunodeficiency

Článek Herpes zoster in the Czech Republic – Epidemiology and Clinical Manifestations

Článek A Simple Method for the Detection of CD154 (CD40L) on Peripheral Blood Lymphocytes

Článok vyšiel v časopise

Epidemiology, Microbiology, Immunology

2010 Číslo 3

Najčítanejšie v tomto čísle