Kissová R., Maďarová L., Klement C.: Laboratory Diagnosis of Pandemic Influenza at the Department of Medical Microbiology of the Regional Authority of Public Health Based in Banská Bystrica in the Season 2009–2010
Authors:
R. Kissová; L. Maďarová; C. Klement
Authors place of work:
Regionálny úrad verejného zdravotníctva so sídlom v Banskej Bystrici, Odbor lekárskej mikrobiológie
Published in the journal:
Epidemiol. Mikrobiol. Imunol. 60, 2011, č. 1, s. 32-37
Summary
The Department of Medical Microbiology of the Regional Authority of Public Health (RAPH) in Banská Bystrica serves as a catchment laboratory of virology for the Central Slovakia Region, and in the influenza season 2009/10, it also served as such for the East Slovakia Region.
Material and methods:
Specimens (nasopharyngeal swabs and post-mortem specimens) from patients with suspected influenza were obtained from both sentinel and non-sentinel physicians. The specimens were analyzed by a rapid test, followed by real-time PCR (RT-PCR) for influenza A or B diagnosis. RT-PCR subtyping for pandemic influenza A/H1N1 was performed.
Results:
From May 2009 to June 2010, 2497 specimens were analyzed for the presence of influenza A and B viruses and in particular for the presence of pandemic influenza A/H1N1 virus. As many as 537 of 589 influenza A-positive specimens, i.e. 21.5% of all specimens analyzed and 91.2% of influenza A-positive specimens, were subtyped as pandemic influenza A/H1N1.
Conclusion:
In the influenza season 2009/10, the new pandemic influenza A/H1N1 clearly predominated in Central and Eastern Slovakia. PCR tests have played a key role in diagnosing patients with suspected pandemic influenza in the laboratory participating in the surveillance of influenza and influenza-like illness in the Slovak Republic.
Keywords:
pandemic influenza A/H1N1 – respiratory viruses – PCR diagnosis – influenza surveillance.
Zdroje
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Štítky
Hygiene and epidemiology Medical virology Clinical microbiologyČlánok vyšiel v časopise
Epidemiology, Microbiology, Immunology
2011 Číslo 1
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