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IGRA methods in the routine operation – QuantiFERON®-TB Gold or T-SPOT.TB?


Authors: J. Amlerová;  J. Hrabák
Authors place of work: Ústav mikrobiologie a Biomedicínské centrum, Lékařská fakulta a Fakultní nemocnice v Plzni, Univerzita Karlova
Published in the journal: Epidemiol. Mikrobiol. Imunol. 65, 2016, č. 4, s. 246-248
Category: Short Communication

Summary

For indirect diagnosis of tuberculosis, two commercial IGRA (Interferon Gamma Release Assay) assays are available – primal QuantiFERON®-TB Gold test, new version QuantiFERON®-TB Gold Plus test (four tube, differentiation in activity CD4+ a CD8+) and T-SPOT®.TB test. Both methods are based on the same principle, but their workflows are different. In this article, both assays are compared on the collection of 284 patients. Inter-rate agreement measure showed 81.3% consistency and Cohen’s kappa index was calculated as 0.72. In case of discrepancy between IGRA and other methods (clinical aspects, X-ray diagnostic, etc.), results should be confirmed by second IGRA assay for correct interpretation.

KEYWORDS:
IGRA – Mycobacterium tuberculosis – Quantiferon – T-SPOT®.TB


Zdroje

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Štítky
Hygiene and epidemiology Medical virology Clinical microbiology
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