Possibilities for the analysis of peripheral blood B cell subpopulations in a routine immunological laboratory
Authors:
J. Štíchová 1; J. Nechvátalová 1,2; J. Litzman 1,2; M. Vlková 1,2
Authors place of work:
Ústav klinické imunologie a alergologie, LF MU v Brně
1; Ústav klinické imunologie a alergologie, Fakultní nemocnice u sv. Anny v Brně, Brno
2
Published in the journal:
Epidemiol. Mikrobiol. Imunol. 70, 2021, č. 4, s. 264-280
Category:
Review Article
Summary
B cells play a vital role in the defence of the body against infectious agents. Apart from their ability to present antigen to T cells, B cells are mainly producers of antibodies. These play a crucial role in the effective elimination of infection and are also involved in the regulation of the immune response. The analysis of peripheral blood B cell subpopulations that makes it possible to monitor the development of B cells to the stage of antibody producing plasmablasts provides a valuable laboratory parameter which is important for both the study of the pathogenesis and diagnosis of some diseases. Laboratory analysis of B cell subpopulations is now a routinely available laboratory option thanks to the development of multicolour flow cytometry. This article summarizes the core knowledge which is currently applied to the analysis of B cell subpopulations in immunological laboratories.
Keywords:
B cell– flow cytometry – marginal zone of the spleen – germinal centre
Zdroje
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Štítky
Hygiene and epidemiology Medical virology Clinical microbiologyČlánok vyšiel v časopise
Epidemiology, Microbiology, Immunology
2021 Číslo 4
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