Screening of benzodiazepines in urine by liguid chromatography with tandem mass spectrometric detection
Authors:
L. Chytil 1,2; V. Marešová 1; O. Slanař 2
Authors place of work:
Ústav soudního lékařství a toxikologie 1. LF UK a VFN
1; Farmakologický ústav 1. LF UK
2
Published in the journal:
Soud Lék., 56, 2011, No. 1, p. 10-16
Category:
Original Article
Summary
A sensitive and selective method for screening of benzodiazepines and their metabolites in urine using liquid chromatography coupled with tandem mass spectrometric detection is presented. Analytes were separated on C18 column using gradient elution. Ionisation of analytes was performed by positive electrospray operated in Selected Reaction Monitoring mode. Optimization of the chromatographic method, acid hydrolysis and liquid – liquid extraction was also described. The limit of detection for most of analytes was 1 ng/mL. The chromatograms of real samples, which are also presented, demonstrate the selectivity, universality and simplicity of the developed method.
Key words:
Benzodiazepines - LC-MS/MS - acid hydrolysis - liquid–liquid extraction - biotransformation.
Zdroje
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Štítky
Anatomical pathology Forensic medical examiner ToxicologyČlánok vyšiel v časopise
Forensic Medicine
2011 Číslo 1
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