PCR diagnosis of infectious diseases

Česká verzia

Authors: Tomáš Freiberger
Authors place of work: Centrum kardiovaskulární a transplantační chirurgie, Brno
Published in the journal: Vnitř Lék 2017; 63(7-8): 472-474
Category: Reviews

Summary

The practices based on microbe cultivation are the corner stone of laboratory diagnostics of infectious diseases etiology. However, cultivation-independent molecular methods for microorganism detection are increasingly being used in a routine clinical setting as an important complement to the conventional procedures and, in some cases, they have already become the golden diagnostic standard. The PCR-based methods are used the most frequently.

Key words:
molecular genetic detection of microorganisms – polymerase chain reaction (PCR)

Zdroje

1. Kolek M, Žaloudíková B, Freiberger T et al. Infekční endokarditida aortální a mitrální chlopně vyvolaná Tropheryma whipplei při nepřítomnosti gastrointestinálních projevů Whippleovy choroby – kazuistika. Klin Mikrobiol Infekc Lek 2007; 13(5): 212–215.

2. Li JS, Sexton DJ, Mick N et al. Proposed modifications to the Duke criteria for the diagnosis of infective endocarditis. Clin Infect Dis 2000; 30(4): 633–638. Dostupné z DOI: <https://doi.org/10.1086/313753>.

3. Vaněrková M, Žaloudíková B, Němcová E et al. Detection of Cardiobacterium valvarum in a patient with aortic valve infective endocarditis by broad-range PCR. J Med Microbiol 2010; 59(Pt 2): 231–234. Dostupné z DOI: <http://dx.doi.org/10.1099/jmm.0.012948–0>.

4. Freiberger T. Molekulární detekce patogenů. In: Souček M et al (ed). Vnitřní lékařství. Grada: Praha-Brno 2011: 52–54. ISBN 978–80–247–2110–1. 978–80–904731–0–2 a 978–80–210–5418–9.