Pre-cooled gel sleeves as a useful tool for preparation of the hematopoietic stem cells before cryoconservation
Authors:
B. Kubešová; J. Vinklárková; J. Komárková; D. Procházková; E. Matějková
Authors place of work:
Tkáňová banka, Fakultní nemocnice Brno
Published in the journal:
Transfuze Hematol. dnes,16, 2010, No. 2, p. 78-81.
Category:
Comprehensive Reports, Original Papers, Case Reports
Summary
The regular course for cell cryoconservation is very important for further condition of cells after thawing. The obtaining of vital cells and in maximum rate is notable especially for hematopoietic stem cell transplantation. On Hematopoietic Cell Department of Tissue Bank in University Hospital Brno a new method of cell preparing for cryoconservation using special designed gel casing for bags was established. Pre-cooling of these casings minimizes the heat-damage of the cells during their mixture with cryoprotective solution which contains dimethylsulfoxid (DMSO). The cord blood obtained from healthy volunteers was used as a material in this experiment. In following article the authors describe the results of comparative study, in which comparing of traditional method of cell preparation using iced water bath and new approach with pre-cooled gel casing was performed.
Key words:
cryoconservation, stem cells, cord blood
Zdroje
1. Jeurink PV, Vissers YM, Rappard B, Savelkoul HFJ. T cell responses in fresh and cryopreserved peripheral blood mononuclear cells: Kinetics of cell viability, cellular subsets, proliferation, and cytokine production Cryobiology 2008; 57: 91-103.
2. Hernández-Navarro F, Ojeda E, Arrieta, R, et al. Hematopoietic cell transplantation using plasma and DMSO without HES, with non-programmed freezing by immersion in a methanol bath: results in 213 cases. Bone Marrow Transplantation 1998; 21: 511-517.
3. Muldrew K, Schachar J, Cheng P, Rempel C, Liang S, Wan R. The possible influence of osmotic poration on cell membrane water permeability. Cryobiology 2009; 58: 62-68.
Štítky
Haematology Internal medicine Clinical oncologyČlánok vyšiel v časopise
Transfusion and Haematology Today
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