A case report of a cystic fibrosis patient with repeated isolation of Trichosporon mycotoxinivorans identified by a novel short-extraction method

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Background:
Trichosporon mycotoxinivorans is a recently described yeast-like fungal organism and its association as a pathogen for patients with cystic fibrosis (CF) was reported previously. We show the clinical course of a CF patient over 9 years as well as the applications of modern molecular and proteomic identification techniques of this rare fungus.

Case presentation:
We present the case of a 32-year-old male CF patient with sputum cultures continuously positive with the anamorphic yeast T. mycotoxinivorans during 9 years. Furthermore, susceptibility testing of T. mycotoxinivorans to different antifungals were performed. In addition, a rapid identification method of this novel fungal pathogen with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was applied using a simple extraction protocol.

Conclusions:
Our case presentation confirms T. mycotoxinivorans as a potential emerging pathogen in patients with CF. However, our CF patient showed mild symptoms over a very long time period of 9 years. A short MALDI-TOF MS procedure allows reliable and rapid identification of T. mycotoxinivorans and therefore should facilitate further study on the clinical relevance and epidemiology of this unusual fungal organism.

Keywords:
Trichosporon mycotoxinivorans, Cystic fibrosis (CF), Internal transcribed spacer (ITS), Emerging pathogen, MALDI-TOF MS

Autoři: Daniel Goldenberger† ^1*; Vladimira Hinić† ¹; Spasenija Savic Prince ²; Michael Tamm ³; Anna-Maria Balestra ^3,4; Doris Hohler ¹; Reno Frei ¹
Působiště autorů: Division of Clinical Microbiology, University Hospital Basel, Petersgraben , CH-40 1 Basel, Switzerland. ¹; Division of Pathology, University Hospital Basel, Schönbeinstrasse 0, CH-40 1 Basel, Switzerland. ²; Division of Pneumology, University Hospital Basel, Petersgraben , CH- 031 Basel, Switzerland. ³; Present address: Division of Pneumology, St. Claraspital Basel, Basel, Switzerland. ⁴
Vyšlo v časopise: BMC Infectious diseases 2016, 16:601
Kategorie: Case report
prolekare.web.journal.doi_sk: https://doi.org/10.1186/s12879-016-1910-7

© 2016 The Author(s).

Open access
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
The electronic version of this article is the complete one and can be found online at: http://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-016-1910-7

Souhrn

Keywords:
Trichosporon mycotoxinivorans, Cystic fibrosis (CF), Internal transcribed spacer (ITS), Emerging pathogen, MALDI-TOF MS

Zdroje

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