In vitro reaktivace acetylcholinesterázy inhibované O-isopropylmethylfluorofosfonátemužitím biskvartérního oximu HS-6

Byla vyhodnocena schopnost oximu HS-6 [1-(4-hydroxyiminomethylpyridinium)-3-(4-karbamoylpyridinium)-2-oxa-propan dichlorid] in vitro reaktivovat enzym acetylcholinesterázu inhibovanýnervově paralytickou látkou – sarinem [O-isopropylmethylfluorofosfonát]. Jako referenční látkybyly vybrány reaktivátory 2-PAM [2-hydroxyiminomethyl-1-methylpyridinium chlorid], obidoxim[1,3-bis(4-hydroxyiminomethylpyridinium)-2-oxa-propan dichlorid] a H-oxim HI-6 [1-(2-hydroxyiminomethylpyridinium)-3-(4-karbomoylpyridinium)-2-oxa-propan dichlorid]. Oxim HS-6 se ukázaljako účinný reaktivátor AChE inhibované sarinem. Nedosahuje sice účinnosti oximu jako H-oximHI-6, nicméně je lepší než 2-PAM či obidoxim.

Klíčová slova:
acetylcholinesteráza – sarin – HS-6 – HI-6 – obidoxim – 2-PAM – in vitro

The in vitro Reactivation of O-Isopropylmethylfluorophosphonate – Inhibited Acetylcholinesteraseby Bisquaternary Oxime HS-6

The ability of the oxime HS-6 [1-(4-hydroxyiminomethylpyridinium)-3-(4-carbamoylpyridinium)-2--oxa-propane dichloride] to reactive in vitro the enzyme acetylcholinesterase inhibited by the nerveagent sarin [O-isopropylmethylfluorophosphonate] was evaluated. The reactivators 2-PAM [2-hydroxyiminomethyl-1-methylpyridinium chloride], toxogonin [1,3-bis(4-hydroxyiminomethylpyridinium)-2-oxa-propane dichloride] and H-oxime HI-6 [1-(2-hydroxyiminomethylpyridinium)--3-(4-carbamoylpyridinium)-2-oxa-propane dichloride] were chosen for comparison. The oxime HS-6was an effective reactivator of sarin-inhibited AChE. It is not as effective as the H-oxime HI-6, butit is better than 2-PAM and obidoxime.

Key words:
acetylcholinesterase – sarin – HS-6 – HI-6 – obidoxime – 2-PAM – in vitro

Autoři: K. Kuča; J. Cabal
Působiště autorů: Katedra toxikologie Vojenské lékařské akademie Jana Evangelisty Purkyně, Hradec Králové
Vyšlo v časopise: Čes. slov. Farm., 2004; , 93-95
Kategorie: Články