Effect of Long-Term Consumption of Lactobacillus paracasei SD1 on Reducing Mutans streptococci and Caries Risk: A Randomized Placebo-Controlled Trial
Background:
A previous study revealed Lactobacillus paracasei SD1, a probiotic strain, could reduce mutans streptococci (MS). The aim of this study was to evaluate the long-term effects of L. paracasei SD1 on the colonization of MS, and whether caries lesions developed.
Methods:
After informed consent, 122 children were recruited and randomly assigned to the probiotic or control groups. The probiotic group received milk-powder containing L. paracasei SD1 and the control group received standard milk-powder once daily for six months. Salivary MS and lactobacilli were enumerated using differential culture at baseline and at three-month intervals for 12 months. The persistence of L. paracasei SD1 was investigated using AP-PCR for DNA-fingerprinting. Oral health was examined at baseline and at the end of the study according to WHO criteria.
Results:
The long-term consumption could prolong colonization of L. paracasei SD1. Significantly reduced MS counts and increased lactobacilli levels were found among children in the probiotic group. There were less caries lesions in the probiotic group at the end of the study. A significant reduction of the development of new caries lesions (4.5 times) was observed in the high caries risk group but not in the low caries risk group.
Conclusions:
Results demonstrate that the long-term daily ingestion of the human-derived probiotic L. paracasei SD1 significantly reduces the number of MS and caries risk in the high caries group.
Keywords:
Lactobacillus paracasei SD1; mutans streptococci; probiotics; dental caries
Autoři:
Rawee Teanpaisan 1,*; Supatcharin Piwat 2; Sukanya Tianviwat 2; Benchamat Sophatha 1; Thanyanan Kampoo 1
Působiště autorů:
Common Oral Diseases and Epidemiology Research Center and the Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat-Yai 9011 , Thailand
1; Common Oral Diseases and Epidemiology Research Center and the Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat-Yai 90112, Thailand
2
Vyšlo v časopise:
Dent. J.2015 3(2)
Kategorie:
Article
prolekare.web.journal.doi_sk:
https://doi.org/10.3390/dj3020043
© 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The electronic version of this article is the complete one and can be found online at: http://www.mdpi.com/2304-6767/3/2/43
Souhrn
Background:
A previous study revealed Lactobacillus paracasei SD1, a probiotic strain, could reduce mutans streptococci (MS). The aim of this study was to evaluate the long-term effects of L. paracasei SD1 on the colonization of MS, and whether caries lesions developed.
Methods:
After informed consent, 122 children were recruited and randomly assigned to the probiotic or control groups. The probiotic group received milk-powder containing L. paracasei SD1 and the control group received standard milk-powder once daily for six months. Salivary MS and lactobacilli were enumerated using differential culture at baseline and at three-month intervals for 12 months. The persistence of L. paracasei SD1 was investigated using AP-PCR for DNA-fingerprinting. Oral health was examined at baseline and at the end of the study according to WHO criteria.
Results:
The long-term consumption could prolong colonization of L. paracasei SD1. Significantly reduced MS counts and increased lactobacilli levels were found among children in the probiotic group. There were less caries lesions in the probiotic group at the end of the study. A significant reduction of the development of new caries lesions (4.5 times) was observed in the high caries risk group but not in the low caries risk group.
Conclusions:
Results demonstrate that the long-term daily ingestion of the human-derived probiotic L. paracasei SD1 significantly reduces the number of MS and caries risk in the high caries group.
Keywords:
Lactobacillus paracasei SD1; mutans streptococci; probiotics; dental caries
Zdroje
1. Meurman, J.H.; Stamatova, I. Probiotics: contributions to oral health. Oral Dis 2007, 13, 443–451.
2. Cagetti, M.G.; Mastroberardino, S.; Milia, E.; Cocco, F.; Lingström, P.; Campus, G. The use of probiotic strains in caries prevention: a systematic review. Nutrients 2013, 5, 2530–2550.
3. Simark-Mattsson, C.; Emilson, C.G.; Hakansson, E.G.; Jacobsson, C.; Roos, K.; Holm, S. Lactobacillus.-mediated interference of mutans streptococci in caries-free vs. caries-active subjects. Eur J. Oral Sci. 2007, 115, 308–314.
4. Strahinic, I.; Busarcevic, M.; Pavlica, D.; Milasin, J.; Golic, N.; Topisirovic, L. Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates. Oral Microbiol. Immunol. 2007, 22, 111–117.
5. Teanpaisan, R.; Piwat, S.; Dahlen, G. Inhibitory effect of oral Lactobacillus against oral pathogens. Lett. Appl. Microbiol. 2011, 53, 452–459.
6. Adams, M.R.; Martean, P. On the safety of lactic acid bacteria from food. Int J. Food. Microbiol. 1995, 27, 263–264.
7. Yli-Knuuttila, H.; Snall, J.; Kari, K.; Meurman, J.H. Colonization of Lactobacillus rhamnosus GG in the oral cavity. Oral Microbiol. Immunol. 2006, 21, 129–131.
8. Caglar, E.; Topcuoglu, N.; Cildir, S.K.; Sandalli, N.; Kulekci, G. Oral colonization by Lactobacillus reuteri ATCC 55730 after exposure to probiotics. Int. J. Paediatr. Dent. 2009, 19, 377–381.
9. Ravn, I.; Dige, I.; Meyer, R.L.; Nyvad, B. Colonization of the oral cavity by probiotic bacteria. Caries Res. 2012, 46, 107–112.
10. Teanpaisan, R.; Piwat, S. Lactobacillus paracasei SD1, a novel probiotic, reduces mutans streptococci in human volunteers: a randomized placebo-controlled trial. Clin. Oral Investig. 2014, 18, 857–862.
11. Ritthagol, W.; Saetang, C.; Teanpaisan, R. Effect of probiotics containing Lactobacillus paracasei SD1 on salivary mutans streptococci and lactobacilli in orthodontic cleft patients: A double-blinded, randomized, placebo-controlled study. Cleft Palate Craniofac. J. 2014, 51, 257–263.
12. Nase, L.; Hatakka, K.; Savilahti, E.; Saxelin, M.; Ponka, A.; Poussa, T.; Korpela, R.; Meurman, J.H. Effect of long-term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries Res. 2001, 35, 412–420.
13. Cildir, S.K.; Germec, D.; Sandalli, N.; Ozdemir, F.I.; Arun, T.; Twetman, S.; Caglar, E. Reduction of salivary mutans streptococci in orthodontic patients during daily consumption of yoghurt containing probiotic bacteria. Eur. J. Orthod. 2009, 31, 407–411.
14. Cildir, S.K.; Sandalli, N.; Nazli, S.; Alp, F.; Caglar, E. A novel delivery system of probiotic drop and its effect on dental caries risk factors in cleft lip/palate children. Cleft Palate Craniofac. J. 2012, 49, 369–372.
15. Caglar, E.; Cildir, S.K.; Sandallin, N.; Ergeneli, S.; Twetman, S. Salivary mutans streptococci and lactobacilli levels after ingestion of the probiotic bacterium Lactobacillus reuteri ATCC 55730 by straws or tablets. Acta. Odontol. Scand. 2006, 64, 314–318.
16. Caglar, E.; Kuscu, O.O.; Cildir, S.K.; Kuvvetli, S.S.; Sandallin, N. A probiotic lozenge administered medical device and its effect on salivary mutans streptococci and lactobacilli. Int. J. Paediatr. Dent. 2008, 18, 35–39.
17. Aminabadi, N.A.; Erfanparast, L; Ebrahimi, A.; Oskouei, S.G. Effect of chlorhexidine pretreatment on the stability of salivary lactobacilli probiotic in six- to twelve-year-old children: A randomized controlled trial. Caries Res. 2011, 45, 148–154.
18. Jindal, G.; Pandey, R.K.; Agarwal, J.; Singh, M. A comparative evaluation of probiotics on salivary mutans streptococci counts in Indian children. Eur. Arch. Paediatr. Dent. 2011, 12, 211–215.
19. Parvez, S.; Malik, K.A.; Ah Kang, S.; Kim, H.Y. Probiotics and their fermented food products are beneficial for health. J. Appl. Microbiol. 2006, 100, 1171–1185.
20. Piwat, S.; Teanpaisan, R.; Dahlén, G.; Thitasomakul, S.; Douglas, C.W. Acid production and growth by oral Lactobacillus species in vitro. J. Investig. Clin. Dent. 2012, 3, 56–61.
21. Guidelines for the Evaluation of Probiotics in Food; Joint FAO/WHO Working Group: London, UK, 2002.
22. Wannun, P.; Piwat, S.; Teanpaisan, R. Purification and characterization of bacteriocin produced by oral Lactobacillus paracasei SD1. Anaerobe 2014, 15, 17–21.
23. Teanpaisan, R.; Chooruk, A.; Wannun, A.; Wichienchot, S.; Piwat, S. Survival rates of human-derived probiotic Lactobacillus paracasei SD1 in milk powder using spray drying. Songklanakarin. J. Sci. Technol. 2012, 34, 241–245.
24. World Health Organization. Oral Health Surveys, Basic Methods, 3rd ed.; World Health Organization: Geneva, Switzerland, 1987.
Štítky
StomatológiaČlánok vyšiel v časopise
Dentistry Journal
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2015 Číslo 2
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