Mineralization of Early Stage Carious Lesions In Vitro—A Quantitative Approach

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Micro computed tomography has been combined with dedicated data analysis for the in vitro quantification of sub-surface enamel lesion mineralization. Two artificial white spot lesions, generated on a human molar crown in vitro, were examined. One lesion was treated with a self-assembling peptide intended to trigger nucleation of hydroxyapatite crystals. We non-destructively determined the local X-ray attenuation within the specimens before and after treatment. The three-dimensional data was rigidly registered. Three interpolation methods, i.e., nearest neighbor, tri-linear, and tri-cubic interpolation were evaluated. The mineralization of the affected regions was quantified via joint histogram analysis, i.e., a voxel-by-voxel comparison of the tomography data before and after mineralization. After ten days incubation, the mean mineralization coefficient reached 35.5% for the peptide-treated specimen compared to 11.5% for the control. This pilot study does not give any evidence for the efficacy of peptide treatment nor allows estimating the necessary number of specimens to achieve significance, but shows a sound methodological approach on the basis of the joint histogram analysis.

Keywords:
enamel caries; mineralization; demineralization; self-assembling peptide; image registration; micro computed tomography; joint histogram

Autoři: Hans Deyhle ¹; Iwona Dziadowiec ¹; Lucy Kind ²; Peter Thalmann ¹; Georg Schulz ¹; Bert Müller ^1,*
Působiště autorů: Biomaterials Science Center, University of Basel, Gewerbestrasse 14, 41 3 Allschwil, Switzerland ¹; University of Applied Sciences and Arts Northwestern Switzerland FHNW, Gründenstrasse 40, 4132 Muttenz, Switzerland ²
Vyšlo v časopise: Dent. J.2015 3(4)
Kategorie: Article
prolekare.web.journal.doi_sk: https://doi.org/10.3390/dj3040111

© 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
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The electronic version of this article is the complete one and can be found online at: http://www.mdpi.com/2304-6767/3/4/111

Souhrn

Keywords:
enamel caries; mineralization; demineralization; self-assembling peptide; image registration; micro computed tomography; joint histogram

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