Raman label-free visualisation of Titanium dioxide nanoparticles uptake in BJ cell LINES
Titanium dioxide nanoparticles represent one of the most frequently applied nanomaterials. Due to its advantageous physicochemical properties affecting the final products, use of this nanomaterial in daily used products is increasing. Beside the addition into glaze or enamels, titanium dioxide nanoparticles are found in UV protective cosmetic products applied on skin. According to the studies confirming the potential carcinogenic effect of titanium dioxide nanoparticles application of such nanomaterial may cause health risk. Cellular uptake of nanoparticles and their distribution in cell environment may play an important role in nanoparticles toxicological effect. Thus, evaluation of cellular uptake of nanoparticles is the additional step for evaluation of nanoparticles toxicology. The main objective of this study was to confirm the assumption of the cellular uptake of tested TiO2 nanoparticles using human fibroblasts BJ cell lines and confocal Raman microscopy as a new, promising, label-free imaging technique for studying the distribution of exogenous substances in cells. The results of this study confirm that tested TiO2 nanoparticles are uptaken by cells and distributed in intracellular environment, where form aggregates, possibly during their transport via endocytosis.
Keywords:
titanium dioxide nanoparticles, cellular uptake, label-free, confocal Raman microscopy
Autoři:
Monika Harvanová 1; Vlastimil Mašek 1; Dagmar Jirova 2; Hana Kolářová 3
Působiště autorů:
Department of Pharmacology, Institute of Molecular and Translational Medicine, Faculty
of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
1; Center of toxicology and Health Safety, National Institute of Public Health, Prague, Czech Republic
2; Department of Medical Biophysics, Institute of Molecular and Translational Medicine, Faculty
of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
3
Vyšlo v časopise:
Lékař a technika - Clinician and Technology No. 1, 2016, 46, 25-28
Kategorie:
Původní práce
Souhrn
Titanium dioxide nanoparticles represent one of the most frequently applied nanomaterials. Due to its advantageous physicochemical properties affecting the final products, use of this nanomaterial in daily used products is increasing. Beside the addition into glaze or enamels, titanium dioxide nanoparticles are found in UV protective cosmetic products applied on skin. According to the studies confirming the potential carcinogenic effect of titanium dioxide nanoparticles application of such nanomaterial may cause health risk. Cellular uptake of nanoparticles and their distribution in cell environment may play an important role in nanoparticles toxicological effect. Thus, evaluation of cellular uptake of nanoparticles is the additional step for evaluation of nanoparticles toxicology. The main objective of this study was to confirm the assumption of the cellular uptake of tested TiO2 nanoparticles using human fibroblasts BJ cell lines and confocal Raman microscopy as a new, promising, label-free imaging technique for studying the distribution of exogenous substances in cells. The results of this study confirm that tested TiO2 nanoparticles are uptaken by cells and distributed in intracellular environment, where form aggregates, possibly during their transport via endocytosis.
Keywords:
titanium dioxide nanoparticles, cellular uptake, label-free, confocal Raman microscopy
Zdroje
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[7] Chernenko, T., Matthäus, C., Milane, L., Quintero, L., Amiji, M., Diem, M., 2009. Label-free Raman spectral imaging of intracellular delivery and degradation of polymeric nanoparticle systems. ACS Nano 3, 3552–3559.
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[9] Shah, N. B., J. Dong, Bischof, J. C., 2011. Cellular uptake and nanoscale localization of gold nanoparticles in cancer using label-free confocal Raman microscopy. Mol. Pharm. 8, 176-184.
[10] IARC: Cobalt in hard metals and cobalt sulfate, galium arsenide, indium phosphide and vanadium pentoxide, 2006, Sci. Publ., 86.
Štítky
BiomedicínaČlánok vyšiel v časopise
Lékař a technika
2016 Číslo 1
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