Endogenous Mouse Dicer Is an Exclusively Cytoplasmic Protein

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Dicer has first been described as the enzyme dedicated to the generation of small RNA fragments from long double stranded RNA. This function of Dicer of playing a central role in the microRNA and short-interfering RNA biogenesis pathways was found to be taking place in the cytoplasm of the cell. However, recent studies reported additional functions of Dicer in the nucleus of human and mouse cell lines, where the protein was proposed to be involved in processing nuclear RNAs as well as in influencing the chromatin state. Consequently, the localization of Dicer within the cell has been highly debated ever since. In this study, we show by biochemical and microscopy techniques that Dicer is only localized in the cytoplasm of embryonic and adult tissues of the mouse. We also exclude the possibility that Dicer only transiently enters the cell nucleus. Our data indicate that nuclear RNA processing is not a conserved feature of mammalian Dicer and highlight the fact that findings from in vitro experiments in cell lines do not always predict the in vivo state within a living organism.

Vyšlo v časopise: Endogenous Mouse Dicer Is an Exclusively Cytoplasmic Protein. PLoS Genet 12(6): e32767. doi:10.1371/journal.pgen.1006095
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1006095

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