Reduction of mRNA export unmasks different tissue sensitivities to low mRNA levels during Caenorhabditis elegans development

English version

Autoři: Angelina Zheleva ^aff001; Eva Gómez-Orte ^aff001; Beatriz Sáenz-Narciso ^aff001; Begoña Ezcurra ^aff001; Henok Kassahun ^aff002; María de Toro ^aff001; Antonio Miranda-Vizuete ^aff004; Ralf Schnabel ^aff005; Hilde Nilsen ^aff002; Juan Cabello ^aff001
Působiště autorů: CIBIR (Center for Biomedical Research of La Rioja), Logroño, La Rioja, Spain ^aff001; Department of Clinical Molecular Biology, Institute of Clinical Medicine, University of Oslo and Akershus University Hospital, Lørenskog, Norway ^aff002; South-Eastern Norway Regional Health Authority, Hamar, Norway ^aff003; Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Sevilla, Spain ^aff004; Institute of Genetics, Technische Universität Braunschweig, Germany ^aff005
Vyšlo v časopise: Reduction of mRNA export unmasks different tissue sensitivities to low mRNA levels during Caenorhabditis elegans development. PLoS Genet 15(9): e32767. doi:10.1371/journal.pgen.1008338
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1008338

Souhrn

Animal development requires the execution of specific transcriptional programs in different sets of cells to build tissues and functional organs. Transcripts are exported from the nucleus to the cytoplasm where they are translated into proteins that, ultimately, carry out the cellular functions. Here we show that in Caenorhabditis elegans, reduction of mRNA export strongly affects epithelial morphogenesis and germline proliferation while other tissues remain relatively unaffected. Epithelialization and gamete formation demand a large number of transcripts in the cytoplasm for the duration of these processes. In addition, our findings highlight the existence of a regulatory feedback mechanism that activates gene expression in response to low levels of cytoplasmic mRNA. We expand the genetic characterization of nuclear export factor NXF-1 to other members of the mRNA export pathway to model mRNA export and recycling of NXF-1 back to the nucleus. Our model explains how mutations in genes involved in general processes, such as mRNA export, may result in tissue-specific developmental phenotypes.

Klíčová slova:

Biology and life sciences – Cell biology – Genetics – Epigenetics – Gene expression – Biochemistry – Nucleic acids – Organisms – Eukaryota – Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Cell processes – Cell cycle and cell division – Animals – Invertebrates – Animal models – Developmental biology – Anatomy – Medicine and health sciences – Cellular structures and organelles – RNA – Embryology – Embryos – Nematoda – Caenorhabditis – Caenorhabditis elegans – RNA interference – Genetic interference – Messenger RNA – Cytoplasm – Digestive system – Pharynx – Respiratory system – Reproductive system – Genital anatomy – Gonads

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