Monitoring of DNA methylation in ovarian cancer using microarrays.

Česká verzia

Authors: M. Chmelařová ¹; E. Ruszová ²; J. Laco ³; E. Dvořáková ⁴; J. Smetana ⁵ ; K. Hrochová ¹; V. Palička ¹
Authors place of work: Ústav klinické biochemie a diagnostiky, Fakultní nemocnice Hradec Králové ¹; Oddělení lékařské genetiky, Fakultní nemocnice Hradec Králové ²; Fingerlandův ústav patologie, Fakultní nemocnice Hradec Králové ³; Porodnická a gynekologická klinika, Fakultní nemocnice Hradec Králové ⁴; Babákova Myelomová skupina, Oddělení patologické fyziologie Masarykova univerzita, Lékařská fakulta ⁵
Published in the journal: Klin. Biochem. Metab., 23 (44), 2015, No. 3, p. 100-104

Summary

Objective:
The aim of this pilot study was whole-genome screening of DNA methylation in ovarian cancer patients. Monitoring of DNA methylation was focused on promoter regions and also on CpG islands.

Design:
Pilot study

Material and methods:
Our study included 6 samples obtained from patients with newly diagnosed ovarian cancer and 6 nonmalignant control samples of ovaries. All samples were analysed using a custom designed methylation microarrays based on the methylated DNA immunoprecipitation.

Results:
Using microarrays designed in collaboration with specialists of the HPST company we found in the tumor tissue tendency to overall DNA hypomethylation. Furthermore we managed to get a set of hypermethylated genes for confirmation analyses.

Conclusion:
DNA methylation changes are considered to be a one of the first changes during carcinogenesis. Due to this fact could be newly identified changes, in case of detection in plasma of ovarian cancer patient, used as screening markers in risk population. Monitoring of DNA methylation could contribute to improve the care of ovarian cancer patients, including possible financial saving due to early diagnosis and possible prediction of response to treatment.

Key words:
ovarian cancer, DNA methylation, microarrays.

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