Potential of Cell‑free Circulating DNA in Diagnosis of Cancer
Authors:
V. Kubaczkova 1,2; L. Sedlaříková 1,2; L. Bešše 1; M. Almaši 1,2
; Hájek R. 1–3; S. Ševčíková 1,2
Authors place of work:
Babákova myelomová skupina, Ústav patologické fyziologie, LF MU, Brno
1; Oddělení klinické hematologie, FN Brno
2; Klinika hematoonkologie LF OU a FN Ostrava
3
Published in the journal:
Klin Onkol 2015; 28(4): 251-259
Category:
Review
doi:
https://doi.org/10.14735/amko2015251
Summary
Circulating cell‑free DNA (cf‑DNA) is characterized as extracellular DNA that may be present in the blood of healthy individuals in low concentrations. Cf‑DNA is released by apoptosis or necrosis into the bloodstream. Increased levels are found in pathological conditions, such as inflammation, autoimmune diseases, or stress. Significant increase of cf‑DNA is particularly evident in patients with malignancies, especially in the advanced stages of the disease. In this case, the tumor specific cf‑DNA is released by necrosis from the cells of primary tumor and metastases. Recently, many studies concentrate on the so‑ called ‘liquid biopsies’ that allow detection of circulating tumor cells and circulating nucleic acids from peripheral blood for tumor diagnostics. Quantitative methods and detection of genetic and epigenetic alternations of cf‑DNA in patients with different malignancies have potential applications in molecular diagnosis, prognosis, monitoring of disease progression and response to treatment. This review focuses on potential utility of cf‑DNA as a blood biomarker in selected solid tumors and hematologic malignancies.
Key words:
circulating cell‑free DNA – tumor marker – solid tumors – hematological malignancies
This study was supported by grant of Internal Grant Agency of the Czech Ministry of Health NT14575.
The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.
The Editorial Board declares that the manuscript met the ICMJE “uniform requirements” for biomedical papers.
Submitted:
13. 3. 2015
Accepted:
18. 5. 2015
Zdroje
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