Wound healing effects after application of polyunsaturated fatty acids in rat

Česká verzia

Authors: A. Hokynková ¹; Z. Wilhelm ²; M. Nováková ²; P. Babula ²; T. Stračina ²; H. Paulová ³; M. Hlaváčová ³; M. Sedláčková ⁴
Authors place of work: Klinika popálenin a plastické chirurgie LF MU a FN Brno ¹; Ústav fyziologie, LF MU a FN Brno ²; Ústav biochemie, LF MU a FN Brno ³; Ústav histologie a embryologie, LF MU a FN Brno ⁴
Published in the journal: Cesk Slov Neurol N 2018; 81(Suplementum 1): 29-31
Category: Original Paper
doi: https://doi.org/10.14735/amcsnn2018S29

Summary

Introduction:

Although mechanisms of polyunsaturated fatty acids influence on skin wound healing have not been fully elucidated yet, it is undisputable that nutritional state affects it profoundly.

Aim:

The study was focused on speed and quality of wound healing influenced by short-lasting oral administration of omega-3 and omega-6 polyunsaturated fatty acids.

Materials and methods:

Young Wistar rats received by orogastric tube either 20% fat emulsion (experimental groups E and F differed by ratio between omega-3 : omega-6 : omega-9 polyunsaturated fatty acids) or water (control group C). After 7 days, skin wound on back was performed and emulsion or water application continued for another week. Blood and tissue samples were obtained during experiment termination. Plasmatic levels of fatty acids, 4-hydroxy-2-nonenal and oxidative stress both in plasma and in wound tissue were examined. Speed and quality of wound healing were assessed by digital planimetry and histological examination. Results were statistically evaluated by non-parametric tests.

Results:

Significant changes of fatty acids plasmatic levels were observed in animals in experimental groups as well as better ability of their plasma and tissues to cope with oxidative stress.

Conclusion:

Short-lasting administration of certain ratio of polyunsaturated fatty acids positively affects skin wound healing.

Key words:

polyunsaturated fatty acids – wound healing – anti-oxidative capacity – 4-HNE – 4-hydroxy-2-nonenal

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manuscript met the ICMJE “uniform requirements” for biomedical papers.

Zdroje

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