Effect of Vanadyl (IV) Sulfate (VOSO₄) on the Production of Flavonoids by the Culture Ononis arvensisL. in vitro
Vliv síranu vanadylu (VOSO₄) na produkci flavonoidů kulturou Ononis arvensis L. in vitro
Byl testován vliv síranu vanadylu (VOSO₄) na produkci flavonoidů v kalusové a suspenzní kultuře Ononis arvensis L. (Fabaceae). Tato látka se osvědčila jako abiotický elicitor pro zvýšení produkce sekundárních látek (flavonoidů) v kultuře Ononis arvensis L. in vitro. Maximální zvýšení tvorby flavonoidů nastalo v kalusové kultuře po 24 hodinové elicitaci VOSO₄ v koncentraci 1,227.10-4 mol/l, a to o 313 % oproti kontrole a v suspenzní kultuře u koncentrace 1,227.10-6 mol/l po 48hodinovém působení elicitoru o 485 % ve srovnání s kontrolou.
Klíčová slova:
Ononis arvensis L. – kalusová a suspezní kultura – flavonoidy – síran vanadylu
Authors:
L. Tůmová; R. Skálová; J. Dušek
Authors place of work:
Univerzita Karlova v Praze, Farmaceutická fakulta v Hradci Králové, Katedra farmakognozie
Published in the journal:
Čes. slov. Farm., 2005; 54, 151-154
Category:
Original Articles
Summary
The study tested the effect of vanadyl (IV) sulfate (VOSO₄) on the production of flavonoids in the callus and suspension culture of Ononis arvensis L. (Fabaceae). This substance proved to be a good abiotic elicitor for an increase in the production of secondary substances (flavonoids) in the culture of Ononis arvensis L. in vitro. The maximal increase in the formation of flavonoids took place in the callus culture after 24-hour elicitation with VOSO₄ in a concentration of 1.227 .10-4 mol/l by 313 % in comparison with the control and in the suspension culture in a concentration of 1.227 .10-6 mol/l after 48-hour action of the elicitor by 485 % in comparison with the control.
Key words:
Ononis arvensis – callus and suspension culture – flavonoids – vanadyl (IV) sulfate
Štítky
Pharmacy Clinical pharmacologyČlánok vyšiel v časopise
Czech and Slovak Pharmacy
2005 Číslo 3
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