Fagopyrum esculentum in vitro
Fagopyrum esculentum in vitro
Pohanka obecná je cenným zdrojem flavonoidu rutinu. Z klíční rostliny byla odvozena kultura in vitro a porovnán obsah flavonoidů v kalusové kultuře a v intaktní rostlině. Pro kultivaci kultury in vitro a produkci flavonoidů je optimální živné medium Murashigeho a Skooga s obsahem kombinace růstových regulátorů 2,4-D 1 mg/l a kinetinu 1 mg/l při kultivaci za normálního světelného režimu.
Klíčová slova:
Fagopyrum esculentum – pohanka – kultura in vitro – rutin – flavonoidy
Authors:
L. Tůmová; M. Píchová; J. Dušek
Authors place of work:
Charles University in Prague, Faculty of Pharmacy Hradec Králové, Czech Republic
Published in the journal:
Čes. slov. Farm., 2007; 56, 125-128
Category:
Original Articles
Summary
Common buckwheat is a valuable source of the flavonoid rutin. Its in vitro culture was derived from a young seedling plant and the content of flavonoids in the callus culture was compared with their content in an intact plant. The optimal nutrient medium for the cultivation of the in vitro culture and for the production of flavonoids is Murashige and Skoog medium which contains, during the cultivation in the normal light regime, the growth regulators combination of 2,4-D 1 mg/l and kinetin 1 mg/l.
Key words:
buckwheat – Fagopyrum – rutin – callus culture
Štítky
Pharmacy Clinical pharmacologyČlánok vyšiel v časopise
Czech and Slovak Pharmacy
2007 Číslo 3
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