Expresion of DD3^PCA3 (Differential Display Code 3) mRNA in tissue of patients with prostate cancer and benign prostatic hyperplasia

Česká verzia

Authors: Jiří Klečka ¹; Milan Hora ¹; Luboš Holubec ³; Martin Pešta ²; Ondřej Topolčan ²; Viktor Eret ¹; Magdalena Chottová-Dvořáková ⁴; Marko Babjuk ⁵; Květoslav Novák ⁵; Josef Stolz ⁵
Authors place of work: Urologická klinika LF UK a FN, Plzeň ¹; Centrální radioizotopová laboratoř LF UK, Plzeň ²; Radioterapeutické oddělení FN a LF UK Plzeň ³; Ústav fyziologie LF UK, Plzeň ⁴; Urologická klinika 1. LF UK a VFN, Praha ⁵
Published in the journal: Ces Urol 2010; 14(1): 39-47
Category: Original article

Summary

Aim:
Early diagnosis of prostate cancer (PCa) in organ confined stage with following radical treatment are only potential currative approach in PCa. Prostatic specific antigen (PSA) is very helpful in early diagnosis, but the main disadvantage is a low positive predictive value, which results in a high number of uselless biopsies. For that reason we need new tests with better parameters. One promising is DD3^PCA3, which is a prostate-specific non-coding mRNA that is highly over-expressed in prostate tumor cells. The aim of study was to evaluate diagnostic potential of DD3^PCA3 for PCa diagnostic.

Material and methods:
We examined altogether 186 patients. In group of patients with suspicion of PCa we collected one tissue specimen core for PCA3 espression in tissue. According to the histologically verification 100 patients with benign prostatic hyperplasia (BPH), and 86 patients with prostate cancer (12 patients from them had prostatic intraepithelial neoplasia (PIN)). Total RNA were isolated, PCA3 and PSA expression quantified using Q RT PCR method. The PCA3/PSA m RNA ratio distribution was determined for both subject groups.

Results:
It was found that levels of mRNA expressions of DD3^PCA3 (Ct) were significantly higher (p < 0.045) in patients with prostate cancer than in patients with benign prostatic hyperplasia. We found no statistically diferencies in patients with prostate cancer in levels of mRNA expressions of DD3^PCA3 (Ct) between patients with organe confined and advanced (metastatic) dissease and according to Gleason score.

Conclusion:
The specificity of mRNA DD3^PCA3 detection seems to be perspective for early differential diagnosis between patients with BPH and patients with prostate cancer.

Key words:
prostate cancer (CaP), benign prostatic hyperplasia (BPH), DD3^PCA3, mRNA, RT PCR

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