Testicular sperm aneu-ploidy in men with spinal cord injury
Authors:
Jiří Heráček 1,2,3; Vladimír Sobotka 1,2,4; Miluše Vozdová 5; Jiří Rubeš 5; Michael Urban 1,2,3; Robert Grill 1,2
Authors place of work:
Univerzita Karlova v Praze, 3. lékařská fakulta, Urologická klinika
1; Fakultní nemocnice Královské Vinohrady, Urologická klinika
2; Androgeos, Praha
3; Pronatal, Praha
4; Výzkumný ústav veterinárního lékařství, v. v. i., Brno
5
Published in the journal:
Ces Urol 2013; 17(1): 36-41
Category:
Original article
Summary
Aim:
The aim of the study was to compare the frequency of numerical abnormalities for chromosomes X, Y, 13, 15, 16, 18, 21 and 22 in men with spinal cord injury to aneuplodia frequency results in normospermic control group of healthy volunteers using fluorescence in situ hybridization.
Method:
Eleven men (aged 21–41 years, average ± SEM: 36.2 ± 11.9) with anejaculation after transversal spinal cord lesions were included into study between 2010 and 2011. Testicular tissue samples with sperms were obtained by microsurgical retrieval and subsequently analysed by fluorescence in situ hybridization. The results were compared with control group of 10 healthy volunteers (aged 26–58 years, average ± SEM: 37.2 ± 13.1) with proved normospermia from ejaculate obtained by masturbation.
Results:
We examined 2613 sperms obtained from testicular tissue in men with spinal cord injury. We found on average 2.95 % of disomic sperms and 4.90 % of diploid sperms for observed chromosomes. Median was 3.54 and 1.63, resp., which shows a larger inter-individual variability in diploid sperm frequency. We examined at least 5000 sperms in each normospermic volunteer. We found 0.62 % of disomic sperms and 0.29 % of diploid sperms (median 1.38 and 0.26, resp.) for observed chromosomes. The frequency of numerical chromosome aberrations was statistically significantly higher in men with spinal cord injury compared with the control group (p < 0.01).
Conclusion:
The study results show high incidence of numerical chromosome aberrations in men with transversal spinal lesion and prove need for genetic work up prior to assisted reproduction.
Key words:
fluorescence in situ hybridization, sperm retrieval, aneuploidy, spinal cord injury.
Zdroje
1. Brackett NL, Lynne CM, Ibrahim E, Ohl DA, Sønksen J. Treatment of infertility in men with spinal cord injury. Nat Rev Urol 2010; 7(3): 162–172.
2. Templado C, Vidal F, Estop A. Aneuploidy in human spermatozoa. Cytogenet Genome Res 2011; 133(2–4): 91–99.
3. World Health Organisation. WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th ed. Geneva: World Health Organization 2010.
4. Schlegel PN. Testicular sperm extraction: microdissection improves sperm yield with minimal tissue excision. Hum Reprod 1999; 14(1): 131–135.
5. Rubes J, Vozdova M, Oracova E, Perreault SD. Individual variation in the frequency of sperm aneuploidy in humans. Cytogenet Genome Res 2005; 111(3–4): 229–236.
6. von Elm E, Altman DG, Egger M, Pocock SJ, Gøtzsche PC, Vandenbroucke JP. The Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement: guidelines for reporting observational studies. J Clin Epidemiol 2008; 61(4): 344–349.
7. Calogero AE, Burrello N, De Palma A, Barone N, D‘Agata R, Vicari E. Sperm aneuploidy in infertile men. Reprod Biomed Online 2003; 6(3): 310–317.
8. Vozdova M, Heracek J, Sobotka V, Rubes J. Testicular sperm aneuploidy in non-obstructive azoospermic patients. Hum Reprod 2012; 27(7): 2233–2239.
9. Gianaroli L, Magli MC, Cavallini G, Crippa A, Nadalini M, Bernardini L, et al. Frequency of aneuploidy in sperm from patients with extremely severe male factor infertility. Hum Reprod 2005; 20(8): 2140–2152.
10. Ferguson KA, Wong EC, Chow V, Nigro M, Ma S. Abnormal meiotic recombination in infertile men and its association with sperm aneuploidy. Hum Mol Genet 2007; 16(23): 2870–2879.
11. Rodrigo L, Rubio C, Peinado V, Villamón R, Al-Asmar N, Remohí J, et al. Testicular sperm from patients with obstructive and nonobstructive azoospermia: aneuploidy risk and reproductive prognosis using testicular sperm from fertile donors as control samples. Fertil Steril 2011; 95(3): 1005–1012.
12. Burrello N, Vicari E, Shin P, Agarwal A, De Palma A, Grazioso C, et al. Lower sperm aneuploidy frequency is associated with high pregnancy rates in ICSI programmes. Hum Reprod 2003; 18(7): 1371–1376.
13. Qiu Y, Wang LG, Zhang LH, Li J, Zhang AD, Zhang MH. Sperm chromosomal aneuploidy and DNA integrity of infertile men with anejaculation. J Assist Reprod Genet 2012; 29(2): 185–194.
14. Nicopoullos J, Gilling-Smith C, Almeida PA, Homa S, Nice L, Tempest H, et al. The role of sperm aneuploidy as a predictor of the success of intracytoplasmic sperm injection? Hum Reprod 2008; 23(2): 240–250.
Štítky
Paediatric urologist Nephrology UrologyČlánok vyšiel v časopise
Czech Urology
2013 Číslo 1
Najčítanejšie v tomto čísle
- Diagnosing adrenal disease – most significant clinical syndromes, biochemical testing and role of imaging methods
- Self-inserting foreign bodies in urogenital tract for the purpose of sexual stimulation
- Life-threatening bleeding into the peritoneal cavity due to spontaneous rupture of angiomyolipoma
- Kidney morcellation after laparoscopic nephrectomy