Prevalence of Glycoprotein B (gB) Genotypes in the Patients with High Risk of Symptomatic Cytomegalovirus Infection
Authors:
K. Roubalová 1
; S. Žufanová 1; A. Vítek 2; M. Staňková 3
Authors place of work:
Centrum laboratorních činností, Státní zdravotní ústav, Praha
1; Ústav hematologie a krevní transfuze, Praha
2; Klinika infekčních nemocí, II. LF UK, Nemocnice Bulovka, Praha.
3
Published in the journal:
Epidemiol. Mikrobiol. Imunol. 58, 2009, č. 4, s. 148-153
Summary
Objectives:
Based on genetic variability of the dominant envelope glycoprotein (gB), human cytomegalovirus is classified into four major genotypes. The aims were to determine the prevalence of particular gB genotypes in Czech CMV-infected patients and to compare three groups of the patients with high risk of symptomatic CMV infection, i.e. haematopoietic stem cell transplant (HSCT) recipients, HIV-positive persons and infants.
Material and methods:
The study was performed on 134 archived CMV-positive DNA isolates from the patients tested in the National Reference Laboratory for Herpesviruses in 2004-2007. For genotyping, the variable part of gB was amplified and analysed using the restriction fragment length polymorphism (RFLP) method.
Results:
The most frequently detected genotype was gB1 (33%), followed by gB2 (29%), gB3 (18%) and gB4 (7%). However, the distribution of gB genotypes varied between groups of high-risk patients: gB2 dominated in HIV-positives (55%, p= 0.004), while gB3 was most common in HSCT recipients (26%, p=0.03) and gB4 was relatively more frequent in infants (20%, p=0.03). In HSCT recipients, we found increased frequency of gB3 (26%, p=0.03) and co-infection with two or more gB genotypes (17%, p=0.016).
Conclusions:
The distribution of CMV gB genotypes from Czech CMV-infected patients is similar to that reported in other European countries or in the United States. Differences in the prevalence of CMV gB genotypes between groups of high-risk patients indicate variation in biological properties of particular gB genotypes, possibly resulting in distinct virulence, immunogenicity or epidemiological characteristics of circulating strains.
Key words:
Human cytomegalovirus – glycoprotein B – genotype – molecular epidemiology.
Zdroje
1. Aquino, V., Figueiredo, L.T. High prevalence of renal transplant recipients infected with more than one cytomegalovirus glycoprotein B genotype. J Med Virol 2000, 61, 138-142.
2. Arista, S., De Grazia, S., Giammanco, G., Di Carlo, P., Iannitto, E. Human cytomegalovirus glycoprotein B genotypes in immunocompetent, immunocompromised, and congenitally infected Italian populations. Arch Virol 2003,148, 547-554.
3. Augutynowicz, E., Gzyl, A., Dzierzanowska, D. Epidemiological studies of HCMV infection in children by restriction analysis of long-PCR products. Acta Microbiol Pol 1999, 48, 341-348.
4. Bale, J.J., Murph, H. J., Demmler, G., Dawson, J., Miller, J., Petheram, S.J. Intrauterine cytomegalovirus infection and glycoprotein B genotypes. J Infect Dis 2000, 182, 933-936.
5. Barbi, M., Corbetta, C., Primache, V., Binda, S., Caroppo, S. et al. CMV gB genotypes and outcome of vertical transmission: study on dried blood spots of congenitally infected babies. J Clin Virol 2001, 21, 75-79.
6. Bongarts, A. ,Von Laer, .D. , Vogelberg, C., Ebert, K.,Van Lunzen, J. et al. Glycoprotein B genotype of human cytomegalovirus: distribution in HIV-infected patients. Scand J Infect Dis 1996, 28, 447-449.
7. Britt, W., Mach, M. Human cytomegalovirus glycoproteins. Intervirology 1996, 39, 401-412.
8. Chern, K., Chandler, D., Martin, D., Kuppermann B., Wolitz R., Margolis, T.P. Glycoprotein B subtyping of cytomegalovirus (CMV) in the vitreous of patients with AIDS and CMV retinitis. J Infect Dis 1998, 178, 1149-1153.
9. Chou, S., Dennison, K.M. Analysis of interstrain variation in cytomegalovirus glycoprotein B sequences encoding neutralization-related epitopes. J Infect Dis 1991, 163, 1229-1234.
10. Coaquette, A., Bourgeois, A., Dirand, C., Varin, A., Chen, W., Herbein, G. Mixed cytomegalovirus glycoprotein B genotypes in immunocompromised patients. Clin Infect Dis 2004, 39, 155-161.
11. Drew, W.L., Chou, S., Miner, R.C., Mohr, B.A., Busch, M.P. et al. Cytomegalovirus glycoprotein B groups in human immunodeficiency virus-infected patients with incident retinitis. J Infect Dis 2002, 186, 114-117.
12. Fidouh-Houhou, N., Duval, X., Bissuel, F., Bourbonneux, V., Flandre, P. et al. Salivary cytomegalovirus (CMV) shedding, glycoprotein B genotype distribution, and CMV disease in human immunodeficiency virus-seropositive patients. Clin Infect Dis 2001, 33, 1406-1411.
13. Gilbert, C., Handfield, J., Toma, E., Lalonde, R., Bergeron, M., Boivin, G. Human cytomegalovirus glycoprotein B genotypes in blood of AIDS patients: lack of association with either the viral DNA load in leukocytes or presence of retinitis. J Med Virol 1999, 59, 98-103.
14. Görzer, I., Kerschner, H., Jaksch, P., Seebacher, G. et al. Virus load dynamics of individual CMV-genotypes in lung transplant recipients with mixed-genotype infections. J Med Virol 2008, 80, 1405-1414.
15. Jin, H., Wang, X., Li S. Human cytomegalovirus glycoprotein B genotype correlates with different symptoms of infected infants. Intervirology 2007, 50, 219-223.
16. Lukácsi, A., Taródi, B., Endreffy, E., Bábinsziki, A., Pál, A., Pusztai, R. Human cytomegalovirus gB genotype 1 is dominant in congenital infections in South Hungary. J Med Virol 2001, 65, 537-542.
17. Meyer-König, U., Vogelberg, C., Bongarts, A., Kampa, D., Delbrück, R. et al. Glycoprotein B genotype correlates with cell tropism in vivo of human cytomegalovirus infection. J Med Virol 1998, 55, 75-81.
18. Meyers, J.D.,Fluornoy, N.,Thomas, E.D. Risc factors for cytomegalovirus infection after human marrow transplantation. J Infect Dis, 1986, 153,478-488.
19. Navarro, D., Lennette, E., Tugizov, S., Pereira, L. Humoral immune response to functional regions of human cytomegalovirus glycoprotein B. J Med Virol 1997, 52, 451-459.
20. Peek, R., Verbraak, F., Bruinenberg, M., Van Der Lelij, A., Van Den Horn, G., Kijlstra, A. Cytomegalovirus glycoprotein B genotyping in ocular fluids and blood of AIDS patients with cytomegalovirus retinitis. Invest Ophthalmol Vis Sci.1998, 39, 1183-7.
21. Pignatelli, S., Dal Monte, P., Rossini, G., Landini, M. Genetic polymorphisms among human cytomegalovirus (HCMV) wild-type strains. Rev Med Virol 2004,14, 383-410.
22. Rasmussen, L., Hong, C., Zipeto D., Morris, S., Sherman, D. et al. Cytomegalovirus gB genotype distribution differs in human immunodeficiencyvirus-infected patients and immunocompromised allograft recipients. J Infect Dis 1997, 175, 179-184.
23. Retiére, C., Lesimple, B., Lepelletier, D., Bignon, J.D., Hallet et al. Association of glycoprotein B and immediate early-1 genotypes with human leukocyte antigen alleles in renal transplant recipients with cytomegalovirus infection. Transplantation 2003, 75, 161-165.
24. Shepp, D., Match, M., Ashraf, A., Lipson, S., Millan, C., Pergollizi, R. Cytomegalovirus glycoprotein B groups associated with retinitis in AIDS. J Infect Dis 1996,174, 184-187.
25. Shepp, D., Match, M., Lipson, S., Pergolizzi, R.G. A fifth human cytomegalovirus glycoprotein B genotype. Res Virol 1998, 149, 109-114.
26. Tarragó, D., Quereda, C., Tenorio, A. Different cytomegalovirus glycoprotein B genotype distribution in serum and cerebrospinal fluid specimens determined by a novel multiplex nested PCR. J Clin Microbiol 2003, 41, 2872-2877.
27. Torok-Storb, B., Boeckh, M., Hoy, C., Leisenring, W., Myerson, D., Gooley, T. Association of specific cytomegalovirus genotypes with death from myelosuppression after marrow transplantation. Blood 1997, 90, 2097-2102.
28. Trincado, D., Scott, G., White, P., Hunt, C., Rasmussen, L., Rawlinson, W.D. Human cytomegalovirus strains associated with congenital and perinatal infections. J Med Virol 2000, 61, 481-487.
29. Vilas Boas, L.S., De Souza, V.A., Penalva De Oliveira, A.C., Rodriguez Viso, A.T., Nascimento Filho, A.M. et al. Cytomegalovirus glycoprotein B genotypes and central nervous system disease in AIDS patients. J Med Virol 2003,71, 404-407.
30. Yamamoto, A.Y., Mussi-Pinhata, M.M., De Deus Wagatsuma, V.M., Marin, L.J., Duarte, G., Figuereido, L.T. Human cytomegalovirus glycoprotein B genotypes in Brazilian mothers and their congenitally infected infants. J Med Virol 2007,79, 1164-1168.
31. Yu, Z.S., Zheng, J.Y., Wu, J.B. Association between clinical manifestations of infants with human cytomegalovirus infection and glycoprotein B genotype. Zhonghua Yi Xue Za Zhi 2007, 87, 259-261.
32. Zhou,U L., Fan, J., Zheng, S.S., Ma, W.H. Genetic variation within the glycoprotein B and H genes of human cytomegalovirus in solid organ transplant recipients. Transpl Infect Dis 2007, 8, 73-77.
33. Zipeto, D., Hong, C., Gerna, G., Zavattoni M., Katzenstein, D. et al. Geographic and demographic differences in the frequency of human cytomegalovirus gB genotypes 1-4 in immunocompromised patients. AIDS Res Hum Retroviruses 1998, 14, 533-536.
Štítky
Hygiene and epidemiology Medical virology Clinical microbiologyČlánok vyšiel v časopise
Epidemiology, Microbiology, Immunology
2009 Číslo 4
Najčítanejšie v tomto čísle
- Antibiotic Resistance and Biofilm Formation in Pseudomonas aeruginosa Strains Isolated from Patients with Urinary Tract Infections
- Dynamics of the Incidence of Creutzfeldt-Jakob Disease in Slovakia in 1975-2008
- Serum Anti-Endomysium Antibodies (AEA) in the Diagnostic Algorithm of Coeliac Disease
- Contribution of HCV core antigen testing in HCV diagnosis by test from the company Abbott Laboratories