Surveillance of West Nile fever in horses in the Czech Republic from 2011 to 2013
Authors:
K. Sedlák 1; H. Zelená 2,3; V. Křivda 1; P. Šatrán 4
Authors place of work:
Oddělení sérologie a virologie, Národní referenční laboratoř pro infekční nemoci koní, Státní veterinární ústav Praha
1; Oddělení virologie, Národní referenční laboratoř pro arboviry, Zdravotní ústav se sídlem v Ostravě
2; Katedra epidemiologie, Fakulta vojenského zdravotnictví, Univerzita obrany, Hradec Králové
3; Oddělení ochrany zdraví zvířat, Ústřední veterinární správa, Státní veterinární správa České republiky
4
Published in the journal:
Epidemiol. Mikrobiol. Imunol. 63, 2014, č. 4, s. 307-311
Category:
Review articles, original papers, case report
Summary
Study aim:
The West Nile virus (WNV) is an important mosquito-borne flavivirus occurring around the world. Occasionally found in Central Europe, the virus spread massively through whole Hungary between 2008 and 2009. The aim of our study was to determine the recent prevalence of the WNV infection in horses in the Czech Republic.
Material and methods:
Overall, 2349 serum samples, collected from healthy unvaccinated adult horses in the Czech Republic between 2011 and 2013, were tested. A commercially available competitive ELISA kit (cELISA) was used for this purpose and positive samples were confirmed by virus neutralisation tests using WNV and tick-borne encephalitis virus (TBEV).
Results:
Altogether 271 of 2348 samples (11.5%) were positive by cELISA. Confirmatory VNT revealed 16 WNV positive samples, 11 of which had titres from 8 to 1024; VNTs with TBEV were negative. Three samples had antibodies against both viruses and the WNV antibody titres were less than or equal to the TBEV antibody titres. A cross reactivity of flaviviruses might have had an impact on the results, but in samples with similar WNV and TBEV titres, co-infection with both pathogens cannot be ruled out either. VNT antibody titres in two horses were inconclusive (cut-off titre 4). The place of birth and transfers (if any) were checked for each WNV seropositive horse. Five WNV positive/TBEV negative samples (0.2 %) came from five administrative regions (South Bohemian, Karlovy Vary, Central Bohemian, South Moravian, and Moravian-Silesian) and the respective animals were never moved to a foreign country. Four of these horses never left the farm. Other six WNV positive/TBEV negative horses were imported to the Czech Republic from North America or Central and West Europe and therefore, it is not possible to tell unambiguously whether their infection is autochthonous or imported.
Conclusion:
The results of the present study confirm that WNV antibodies occur sporadically in horses in the Czech Republic. WNV was found to circulate in different parts of the Czech Republic and not only in the South of Moravia.
Keywords:
West Nile fever – WNV – flaviviruses – serosurveillance – horses
Zdroje
1. Hubálek Z, Halouzka J. West Nile fever-reemerging mosquito borne viral diseases in Europe. Emerg Infect Dis, 1999;5:643–650.
2. Bakonyi T, Ivanics E, Erdélyi K, et al. Lineage 1 and 2 strains of encephalitic West Nile virus, Central Europe. Emerging Infect Dis, 2006;12:618–623.
3. Kutasi O, Bakonyi T, Lecollinet S, et al. Equine encephalomyelitisoutbreak caused by a genetic lineage 2 West Nile virus in Hungary. J Vet Intern Med, 2011;25:586–591.
4. Wodak E, Richter S, Bagó Z, et al. Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009. Vet Microbiol, 2011;149:358–366.
5. May FJ, Davis CT, Tesh RB, et al. Phylogeography of West Nile Virus: from the Cradle of Evolution in Africa to Eurasia, Australia, and the Americas. J Virol, 2011;85(6):2964–2974.
6. Chevalier V, Lecollinet S, Durand B. West Nile virus in Europe: A comparation of surveillance system designs in a changing epidemiological context. Vector Borne Zoonotic Dis, 2011;11:1085–1091.
7. Durand B, Chevallier V, Pouillot R, et al. West Nile virus outbreak in horses, southern France, 2000: Results of a serosurvey. Emerg Infect Dis, 2002;8:777–782.
8. Jimenez-Clavero MA, Tejedor CG, Rojo G, et al. Serosurvey of West Nile Virus in equids and bovids in spain. Vet Rec, 2007;161:212.
9. Autorino GL, Battisti A, Deubel V, et al. West Nile Virus epidemic in horses, Tuscany region, Italy. Emerg Infect Dis, 2002;8:1372–1378.
10. Savuta G, Ionescu A, Dragomir G, et al. Serological investigations of WNV infection in horses from the south-east of Romania. Bull Univ Agricult Sci Vet Med (Cluj Napoca), 2007;64:527–530.
11. Bakonyi T, Ferenzi E, Erdélyi K, et al. Explosive spread of a neuroinvasive lineage 2 West Nile virus in Central Europe, 2008/2009. Vet Microbiol, 2013;165:61–70.
12. Bakonyi T, Hubálek Z, Rudolf I, et al. Novel Flavivirus or New Lineage of West Nile Virus, Central Europe. Emerg Infect Dis, 2005;11:225–231.
13. Hubálek Z, Halouzka J, Juřicová Z, et al. First isolation of mosquito-borne West-Nile virus in the Czech Republic. Acta Virol, 1998;42:119–20.
14. Hubálek Z, Halouzka J, Juřicová Z. West Nile fever in Czechland. Emerg Infect Dis, 1999;5:594–595.
15. Hubálek Z, Halouzka J, Juřicová Z, et al. Surveillance virů přenosných komáry na Břeclavsku v povodňovém roce 1997. Epidemiol Mikrobiol Immunol, 1999;3:91–96.
16. Hubálek Z, Halouzka J, Juřicová Z, et al. Serologic survey of birds for West Nile Flavivirus in Southern Moravia (Czech Republic). Vector Borne Zoonotic Dis, 2008;8:659–666.
17. Hubálek Z, Ludvíková E, Jahn P, et al. West Nile virus Equine serosurvey in the Czech and Slovak Republics. Vector borne and Zoonotic Dis, 2013;13:733–738.
18. Cannon RM. Sense and sensitivity – design survey based on a imperfekt test. Prev Vet Med, 2001;49:141–163.
18. Januška J, Daneš L, Heinz F. Laboratorní vyšetřovací metody arbovirových nákaz a virových nákaz šířených hlodavci. Praha: Avicenum; 1990.
19. Zelená H, Januška J, Raszka J. Micromodification of Virus--neutralisation Assay with Vital Staining in 96-well Plate and its Use in diagnostics of Ťahyňa Virus Infections. Epidemiol Mikrobiol Immunol, 2008;57:106–110.
20. Eiden M, Vina-Rodriguez A, Hoffmann B, et al. Two new real-time quantitative reverse transcription polymerase chain reaction assays with unique target sites for the specific and sensitive detection of lineages 1 and 2 West Nile virus strains. J Vet Diagn Invest, 2010;22:748–53.
21. Ziegler U, Angenvoort J, Klaus Ch, et al. Use of Competition ELISA for monitoring of West Nile virus infections in horses in Germany. Int J Environ Res Public Health, 2013;10:3112–3120.
22. Weissenbock H, Hubálek Z, Halouzka J, et al. Screening for West Nile virus infections in susceptible animal species in Austria. Epidem Infect, 2003;131:1023–1027.
23. Barbic L, Listes E, Katic S, et al. Spreading of West Nile virus infection in Croatia. Vet Microbiol, 2012;159:504–508.
24. Ziegler U, Skrypnyk A, Keller M, Staubach Ch, et al. West Nile virus antibody prevalence in horses of Ukraine. Viruses, 2013;5:2469–2482.
25. Madrid AT, Porterfield JS. The flaviviruses (group B arboviruses): A cross-neutralization study. J Gen Virol, 1974;23:91–96.
26. Calisher CH, Karabatsos N, Dalrymple JM, et al. Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera. J Gen Virol, 1989;70:37–43.
27. Blitwich BJ, Bowen RA, Marlenee NL, et al. Epitope-blocking enzyme-linked immunosorbent assai for detection of West Nile virus antibodies in domestic mammals. J Clin Microbiol, 2003;41:2676–2679.
Štítky
Hygiene and epidemiology Medical virology Clinical microbiologyČlánok vyšiel v časopise
Epidemiology, Microbiology, Immunology
2014 Číslo 4
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