Clonal analysis of the meningococcal populations from invasive disease and healthy carriers isolated in the Czech Republic in 1971–2014 (May)
Authors:
Z. Jandová; M. Musílek; Z. Vacková; J. Kozáková
; P. Křížová
Authors place of work:
Centrum epidemiologie a mikrobiologie, Státní zdravotní ústav, Praha
Published in the journal:
Epidemiol. Mikrobiol. Imunol. 64, 2015, č. 3, s. 147-152
Category:
Original Papers
Summary
Objective:
To present the results of clonal analysis of the meningococcal populations isolated from invasive disease and healthy carriers in the Czech Republic over four decades.
Material and methods:
A total of 2179 isolates of Neisseria meningitidis from 1971–2014 (May) were studied: 1093 isolates from patients with invasive meningococcal disease (IMD) and 1086 isolates from healthy carriers. All study isolates were analysed by multilocus sequence typing (MLST), one of the major methods used in molecular epidemiology of IMD.
Results:
More than 94 % of N. meningitidis isolates from IMD were assigned to serogroups B or C. The strains of the leading serogroup B were genetically highly heterogeneous: 1093 isolates were assigned to 25 clonal complexes. Similarly, the strains of the second leading serogroup C appeared genetically heterogeneous and were classified into 19 clonal complexes. The third leading serogroup Y of IMD isolates showed an opposite tendency and appeared highly homogeneous, with only three clonal complexes being detected. Over 75% of the predominant clonal complexes of IMD isolates of both serogroup Y (cc23) and serogroup C (cc11) were classified as hypervirulent and, as such, posed the highest risk to the host population. Over 80% of IMD isolates of serogroup B were assigned to hypervirulent clonal complexes (cc32, cc41/44, cc18, cc269, and cc11). Of 1086 N. meningitidis isolates from healthy carriers, 41.4% were non-serogroupable, i.e. designated N. meningitidis NG. Classification of these isolates into clonal complexes was highly heterogeneous. In total, 28 clonal complexes were identified of which only a minority were hypervirulent.
Conclusions:
The analysis of MLST data on strains collected over four decades revealed that the population of N. meningitidis strains involved in IMD differ genetically from N. meningitidis strains isolated from healthy carriers. These results are relevant to both the optimal use of preventive measures in a focus of IMD and to the development of an effective meningococcal vaccine and vaccination strategy guidelines.
Keywords:
Neisseria meningitides – multilocus sequence typing – clonal analyse
Zdroje
1. Bratcher HB, Bennett JS, Maiden MC. Evolutionary and genomic in-sights into meningococcal biology. Future Microbiol, 2012; 7(7):873–885.
2. Bronsdon MA, O’Brien KL, Facklam RR, et al. Immunoblot method to detect Streptococcus pneumoniae and identify multiple serotypes from nasopharyngeal secretions. J Clin Microbiol, 2004; 42(4):1596–1600.
3. Cartwright K. Meningococcal carriage and disease. Meningococcal Disease, Chichester, UK: John Wiley & Sons, Ltd; 1995.
4. Cartwright KA, Stuart JM, Jones DM, et al. The Stonehouse survey: nasopharyngeal carriage of meningococci and Neisseria lactamica. Epidemiol Infect, 1987; 99(3):591–601.
5. Caugant DA. Population genetics and molecular epidemiology of Neisseria meningitidis. APMIS, 1998; 106(5):505–525.
6. Caugant DA, Fogg C, Bajunirwe F, et al. Pharyngeal carriage of Neisseria meningitidis in 2-19-year-old individuals in Uganda. Trans R Soc Trop Med Hyg, 2006; 100(12):1159–1163.
7. Caugant DA, Hoiby EA, Magnus P, et al. Asymptomatic carriage of Neisseria meningitidis in a randomly sampled population. J Clin Microbiol, 1994; 32(2):323–330.
8. Caugant DA, Hoiby EA, Rosenqvist E et al. Transmission of Neisseria meningitidis among asymptomatic military recruits and antibody analysis. Epidemiol Infect, 1992; 109(2):241–253.
9. Caugant DA, Kristiansen BE, Froholm LO, et al. Clonal diversity of Neisseria meningitidis from a population of asymptomatic carriers. Infect Immun, 1988; 56(8):2060–2068.
10. Caugant DA, Tzanakaki G, Kriz P. Lessons from meningococcal carriage studies. FEMS Microbiol Rev, 2007; 31(1):52–63.
11. Claus H, Maiden MC, Wilson DJ, et al. Genetic analysis of meningococci carried by children and young adults. J Infect Dis, 2005; 191(8):1263–1271.
12. Hammerschmidt S, Birkholz C, Zahringer U, et al. Contribution of genes from the capsule gene complex (cps) to lipooligosaccharide biosynthesis and serum resistance in Neisseria meningitidis. Mol Microbiol, 1994; 11(5):885–896.
13. Jolley KA, Kalmusova J, Feil EJ, et al. Carried meningococci in the Czech Republic: a diverse recombining population. J Clin Microbiol, 2000; 38(12):4492–4498.
14. Jolley KA, Maiden MC. Automated extraction of typing information for bacterial pathogens from whole genome sequence data: Neisseria meningitidis as an exemplar. Euro Surveill, 2013; 18(4):20379.
15. Krizova P, Kriz B. Factors affecting the occurrence and development of invasive meningococcal disease and development of Neisseria meningitis carrier state – results of a nationwide prospective questionnaire survey of cases and controls. Epidemiol Mikrobiol Imunol, 1999; 48(4):140–152.
16. Krizova P, Musilek M, Vackova Z, et al. Invazivní meningokokové onemocnění v České republice v roce 2013. Zprávy centra epidemiologie a mikrobiologie, 2014; 23:83–89.
17. Krizova P, Roznovsky L. Menigokokové onemocnění, Praha: Maxdorf; 2011.
18. Maiden MC, Bygraves JA, Feil E, et al. Multilocus sequence typing: a portable approach to the identification of clones within popula-tions of pathogenic microorganisms. Proc Natl Acad Sci U S A, 1998; 95(6):3140–3145.
19. Maiden MC, Jansen van Rensburg MJ, Bray JE, et al. MLST revisited: the gene-by-gene approach to bacterial genomics. Nat Rev Microbiol, 2013; 11(10):728–736.
20. Olcen P, Kjellander J, Danielsson D, et al. Epidemiology of Neisseria meningitidis; prevalence and symptoms from the upper respiratory tract in family members to patients with meningococcal disease. Scand J Infect Dis, 1981; 13(2):105–109.
21. Pollard AJ. Global epidemiology of meningococcal disease and vaccine efficacy. Pediatr Infect Dis J, 2004; 23(12 Suppl):S274–279.
22. Stephens DS. Uncloaking the meningococcus: dynamics of carriage and disease. Lancet, 1999; 353(9157):941–942.
23. Tyski S, Grzybowska W, Dulny G, et al. Phenotypical and genotypical characterization of Neisseria meningitidis carrier strains isolated from Polish recruits in 1998. Eur J Clin Microbiol Infect Dis, 2001; 20(5):350–353.
24. Vogel U, Frosch M. Mechanisms of neisserial serum resistance. Mol Microbiol, 1999; 32(6):1133–1139.
25. Yazdankhah SP, Kriz P, Tzanakaki G, et al. Distribution of serogroups and genotypes among disease-associated and carried isolates of Neisseria meningitidis from the Czech Republic, Greece, and Norway. J Clin Microbiol, 2004; 42(11):5146–5153.
Štítky
Hygiene and epidemiology Medical virology Clinical microbiologyČlánok vyšiel v časopise
Epidemiology, Microbiology, Immunology
2015 Číslo 3
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