#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

3,5-Dimethoxyfenol – Marker Intoxication with Taxus baccata


Authors: J. Stříbrný 1,2;  M. Dogoši 1;  Z. Šňupárek 1;  P. Toupalík 3;  P. Baláž 3;  P. Bartoš 3
Authors place of work: Vojenský ústav soudního lékařství ÚVN, U vojenské nemocnice 1200, 169 02 Praha 6, 2Fakulta vojenského zdravotnictví Univerzity obrany, Třebešská 1575, 500 01 Hradec Králové, 3Oddělení soudního lékařství, Pardubická krajská nemocnice, Kyjevská 44, 532 03 P 1
Published in the journal: Soud Lék., 55, 2010, No. 3, p. 36-39

Summary

Autopsy findings of fatal intoxication with yew (Taxus baccata) are nonspecific. A presence of plant residues in the digestive tract can signalize yew intoxication. If yew decoction is consumed, plant residues are not found. In such a case the intoxication can be signalized by the presence of 3,5-dimethoxyphenol in biological material. Authors of this article describe the proof and quantification of the 3,5-dimethoxyphenol in two cases of fatal intoxication with yew. In both cases the liquid/liquid extraction and solid phase extraction was used. Extracts obtained from the acidic and basic environment were analysed. Extracts from the acidic environment were methylated and the extracts from the basic environment were acetylated. The analyses were carried out by gas chromatography/mass spectrometry. In the blood of both intoxicated persons the presence of 3,5-dimethoxyphenol was proved and its concentration 82 ng/ml and 417 ng/ml was quantificated. In both cases the presence of 3,5-dimethoxyphenol was also proved in the gastric contents and urine.

Key words:
3,5-dimethoxyphenol – Taxus baccata – intoxication – GC/MS


Zdroje

1. Beike, J., Karger, B., Meiners, T., Brinkmann, B., Köhler, H.: LC-MS determination of Taxus alkaloids in biological specimens. Int J Legal Med 117, 2003, s. 335–339.

2. Frommherz, L., Kintz, P., Kijewski, H., Köhler, H., Lehr, M., Brinkmann, B., Beike, J.: Quantitative determination of taxine B in body fluids by LC-MS-MS. Int J Legal Med 120, 2006, s. 346–351.

3. Kosatík, A., Smysl, B.: Chemické toxikologické vyšetření při smrtelné otravě tisem. Scripta medica 51, 1978, s. 118–119.

4. Musshoff, F., Jacob, B., Fowinkel, C., Daldrup, T: Suicidal yew leave ingestion — Phloroglucindimethylether (3,5-dimethoxyphenol) as a marker for poisoning from Taxus baccata. Int J Legal Med 106, 1993, s. 45–50.

5. Musshoff, F., Madea, B.: Modern analytical procedures for the determination of taxus alkaloids in biological material. Int. J. Legal Med. 122, 2008, s. 357–358.

6. Pietsch, J., Schulz, K., Schmidt, U., Andresen, H., Schwarze, B., Dreßler, J.: A comparative study of five fatal cases of Taxus poisoning. Int J Legal Med 121, 2007, s. 417–422.

7. Stříbrný, J., Dogoši, M., Šňupárek, Z., Černá, I., Sokol, M.: TLC Proof of Intoxication by Taxus Baccata:. Chem. Listy 101, 2007, s. 275–276.

8. Tekol, Y.: Negative chronotropic and atrioventricular blocking effects of taxine on isolated frog heart and its acute toxicity in mice. Planta Med, 5, 1985, s. 357–360.

9. Tekol, Y., Kameyama, M.: Elektrophysiologische Untersuchungen über den Wirkungsmechanismus des Eibentoxins Taxin auf das Herz. Arzneimittelforschung, 37, 1987, s. 428–431.

10. Vaněrková, H., Lysenková, A.: Toxikologický nález po požití tisu. Folia Fac Med Univ Comenianae Bratisl, Suppl. 1990, s. 157–163.

Štítky
Anatomical pathology Forensic medical examiner Toxicology
Prihlásenie
Zabudnuté heslo

Zadajte e-mailovú adresu, s ktorou ste vytvárali účet. Budú Vám na ňu zasielané informácie k nastaveniu nového hesla.

Prihlásenie

Nemáte účet?  Registrujte sa

#ADS_BOTTOM_SCRIPTS#