Mycobacterium avium subsp. paratuberculosis, etiological factor in Crohn’s disease?

Česká verzia

Authors: B. Fixa ^1,5; I. Pavlík ⁴; J. Bedrna ²; O. Komárková ¹; Z. Nožička ³; I. Slaná ⁴; P. Králík ⁴; T. Vaňásek ⁵; M. Volfová ⁵
Authors place of work: II. interní klinika, LF UK a FN Hradec Králové ¹; Chirurgická klinika, LF UK a FN Hradec Králové ²; Fingerlandův ústav patologické anatomie, LF UK a FN Hradec Králové ³; Výzkumný ústav veterinárního lékařství, v. v. i., Brno ⁴; Hepato-gastroenterologie s. r. o., Hradec Králové ⁵
Published in the journal: Gastroent Hepatol 2010; 64(2): 7-13
Category: Original Article

Summary

Introduction:
The etiology of Crohn’s disease remains open. The significance of Mycobacterium avium subsp. paratuberculosis (MAP) in the development of Crohn’s Disease has for a long time been influenced by the great difficulties associated with the cultivation procedure. The goal of our study was to determine the incidence of MAP by the new highly sensitive PCR method (hsPCR) for the detection of specific DNA fragment IS900 and together with reports in the literature, to assess the position of MAP in the etiology of Crohn’s disease. Material and methods: Small specimens taken from the operated gut of 106 patients with Crohn’s disease were examined for the presence of MAP by a highly sensitive PCR method. Resection of terminal ileum and cecum – primary operations were performed in 61 patients, reoperation in 32 patients, dominant operation of the colon in 11 patients, resection of the jejunum in 2 patients. The results from the involved pathological tissue were compared with the results from macroscopically unaffected tissue (controls). Results: In affected tissue, MAP was present in 58 patients (54.7 %). In 14 of these patients (13.2 %), MAP was also found in control specimens. In 4 patients (3.8 %) MAP was found only in unaffected tissue. In total, MAP was found in 62 patients (58.5 %). Conclusion: A significantly higher incidence of MAP found in the affected tissue of patients with Crohn’s disease in comparison with unaffected tissue supports the view of the possible role of MAP in the etiology of Crohn’s disease.

Key words:
Crohn’s disease – Mycobacterium avium subsp. paratuberculosis – paratuberculosis – Johne‘s disease

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