Multi-marker approach in the diagnostics of cardiac diseases by protein biochip technology
Authors:
M. Vašatová 1; M. Tichý 1; Horáček Jm 2,3; R. Pudil 4; L. Horáková 4; V. Palička 1
Authors place of work:
Univerzita Karlova v Praze, LF a FN Hradec Králové, Ústav klinické biochemie a diagnostiky
1; Univerzita obrany Hradec Králové, Fakulta vojenského zdravotnictví, Katedra válečného vnitřního lékařství
2; Univerzita Karlova v Praze, LF a FN Hradec Králové, II. interní klinika, Oddělení klinické hematologie
3; Univerzita Karlova v Praze, LF a FN Hradec Králové, I. interní klinika
4
Published in the journal:
Čas. Lék. čes. 2009; 148: 591-596
Category:
Original Article
Summary
Background.
Multi-marker approach is recommended for rapid diagnostics and risk stratification of acute coronary syndrome. We tested the analytical performance of protein biochip technology for determination cardiac markers.
Methods. Analysis of cardiac markers:
CK-MB mass, cTnI, myoglobin, glycogen phosphorylase BB (GPBB), heart type of fatty acid binding protein (h-FABP) and carbonic anhydrase III (CAIII) was performed by system Evidence Investigator (Randox). Analytical parameters of Cardiac array were tested. The Evidence Investigator results were compared with Elecsys 2010 (Roche) CK-MB mass and myoglobin methods. Markers of myocardial injury were determined in 28 blood donors, 28 patients with acute myocardial infarction diagnosis and 21 patients after chemotherapy containing anthracyclines (monitoring of cardiotoxicity).
Results.
The Passing-Bablok regression shows statistically significant differences in results. The reasons for these differences are poor standardization of methods and discrepancies between calibrations. New substances h-FABP and GPBB are promising early markers of acute myocardial infarction and diagnostic sensitivity of h-FABP would be better than myoglobin test. These markers can be useful for monitoring of cardiotoxicity of anthracyclines.
Conclusions.
In future, the use of biochip technology in cardiology diagnostic represents an important challenge but it is a necessary standardization of immunochemical methods.
Key words:
protein biochip, cardiac marker, cardiotoxicity, myocardial infarction.
Zdroje
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Štítky
Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain management Dental HygienistČlánok vyšiel v časopise
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