Effects of glass fibers on selected parameters of respiratory tract in experimental conditions

Česká verzia

Authors: Hurbánková Marta ¹; Ďungelová Silvia ¹; Černá Silvia ¹; Tátrai Erzsébet ²; Wimmerová Soňa ¹; Moricová Štefánia ¹
Authors place of work: Slovenská zdravotnícka univerzita, Bratislava, Slovensko, doc. MUDr. Štefánia Moricová, PhD., MPH, mimoriadny profesor, dekanka Fakulty verejného zdravotníctva ¹; National Institute of Environmental Health, Budapešť, Maďarsko ²
Published in the journal: Pracov. Lék., 65, 2013, No. 3-4, s. 113-120.
Category: Original Papers

*Výsledky práce boli prezentované na konferencii „Životné podmienky a zdravie“ v Dolnom Smokovci, 2012.

Summary

Glass fibers are non - crystalline, fibrous inorganic silicates, currently applied in many industrial branches as a substitute of carcinogenic asbestos. Problem of toxicity glass fibers are the subject of many experimental and epidemiological studies. The aim of our study was to find out the effects of glass fibers in lung tissue in terms of inflammatory and cytotoxic parameters of bronchoalveolar lavage (BAL) after exposure of 2, 30, 90 days and results compared with the control group.

Exposed group:
animals were instilled with 4 mg glass fibers in 0.4 ml saline solution/animal, the control group with 0.4 ml saline solution. Wistar rats were sacrificed and bronchoalveolar lavage (BAL) interventions were performed after instillation – 2, 30, 90 days. Following BAL parameters were examined: histology of lung tissues, total count of leukocytes (Le) and alveolar macrophages (AM), differential cell count (% of polymorphonuclears – PMN, % of – AM and % of lymphocytes – Ly ), % immature forms of AM, % binucleated cells, phagocytic activity and viability of AM.

The results of our study indicate that the glass fibers compared with the control group saline significantly affect especially inflammatory parameters in all time points (in the acute – 2 days in the sub-acute phases – 30 and 90 day exposure), which means that even after 90 days after instillation of glass fibers in the lungs of experimental animals still persist and cause an inflammatory reaction.

Keywords:
glass fibers – bronchoalveolar lavage – inflammatory and cytotoxic parameters – intratracheal instillation

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