Expression of Bcl-2 (B-Cell Lymphoma 2) Gene in Primary Pleomorphic Adenoma in Relation to Its Histogenesis
Authors:
K. Obtulovičová 1; I. Stárek 2; A. Skálová 3; A. Kališ 4; M. Sičák 1; P. Čmilanský 5
Authors place of work:
Klinika otorinolaryngológie a chirurgie hlavy a krku, Ústredná vojenská nemocnica a fakultná nemocnica, Ružomberok
1; ORL klinika LF UP a FN v Olomouci
2; Šiklův patologický ústav LF UK, Plzeň
3; Ústav patologickej anatómie, Ústredná vojenská nemocnica a fakultná nemocnica, Ružomberok
4; Poliklinika Sekčov, s. r. o., ORL a SOFYC clinic, Prešov
5
Published in the journal:
Otorinolaryngol Foniatr, 63, 2014, No. 3, pp. 176-181.
Category:
Original Article
Summary
Pleomorphic adenoma is morphologically characterized as biphasic tumour, including epithelial and mesenchymal components. At present its epithelial origin is accepted. Pluripotent unicellular theory is the most plausible tumorigenesis theory. According this theory, the steam cell is the origin for renewal of mature salivary gland tissue as well as for tumour growth. This cell is the basis for epithelial as well as mesenchymal tumour components. Bcl-2 (b-cell lymphoma 2) gene is the steam cell marker that protects cell against apoptosis. In retrospective study, the authors followed up immunoexpression and distribution of bcl-2 oncoprotein in single cell population of 24 primary pleomorphic adenomas. Bcl-2 positivity was detected in 95.8% pleomorphic adenomas (91.7% in epithelial and 62.5% in mesenchymal components). The most intensive immunostaning was found in the outer cells of well differentiated excretory duct resembling formations. The cells of the inner layer were always negative. Week to moderate reaction was present in cells at periphery of excretory-like ductal formations as well as in “intermediate” cells in some unstructured solid regions. The majority of mesenchymal cells were negative, minority showed week to moderate positivity, only occasionally with strong immunoreaction. The occasional foci of squamous cell metaplasia were bcl-2 negative. The study confirmed the pluripotent unicellular theory. Authors consider bcl-2 gene as the key element in pleomorphic adenoma genesis, in cooperation with other antiapoptotic mechanisms.
Keywords:
bcl-2 gene, pleomorphic adenoma, histogenesis, apoptosis
Zdroje
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Štítky
Audiology Paediatric ENT ENT (Otorhinolaryngology)Článok vyšiel v časopise
Otorhinolaryngology and Phoniatrics
2014 Číslo 3
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