NF- κB, Colorectal Carcinoma and Radiotherapy: Results of a Clinical and Experimental Study

Česká verzia

Authors: R. Vobořil ^1,2; J. Weberová-Vobořilová ¹; V. Rychterová ³; M. Ambruš ⁴; L. Horák ²; J. Fanta ²
Authors place of work: Oddělení buněčné a molekulární biologie, Ústav biochemie, buněčné a molekulární biologie, 3. lékařská fakulta UK, Praha, vedoucí oddělení: doc. RNDr. J. Kovář, DrSc. ¹; Chirurgická klinika, 3. lékařská fakulta UK a Fakultní nemocnice Královské Vinohrady, Praha, přednosta: doc. MUDr. J. Fanta, DrSc. ²; Ústav patologie, 3. lékařská fakulta UK a Fakultní nemocnice Královské Vinohrady, Praha, přednosta: prof. MUDr. V. Mandys, CSc. ³; Radioterapeutická a onkologická klinika, 3. lékařská fakulta UK a Fakultní nemocnice Královské Vinohrady, Praha, přednostka: MUDr. M. Kubecová ⁴
Published in the journal: Rozhl. Chir., 2007, roč. 86, č. 5, s. 268-272.
Category: Monothematic special - Original

Summary

Resistance to radiotherapy often seen in rectal cancer patients requires intensive study of mechanisms taking part in this process. It seems that nuclear factor-kappaB (NF- κB), especially its subunit p65, could play an important role in radioresistance. The aims of this study were: 1. to assess expression of p65 in rectal cancer patients and to evaluate its possible predictive role; 2. to determine in in vitro experiments possibilities of p65 modulation and to evaluate, whether this modulation can result in enhacement of efficiency of radiation therapy. We found that the level of NF- κB expression may serve as a predictive marker of overall survival. Further we found that inhibition of NF- κB activity caused by radiation improves impact of radiation therapy.

Key words:
NF-kappaB – rectal cancer – radiotherapy – immunohistochemistry – marker

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