Monitoring of hematopoieticrecovery and cytotoxic activity of NK-cells after allogeneichematopoietic cell transplantation.
Sledovanie obnovy krvotvorby a cytotoxickej aktivityNK-buniek po alogénnej transplantácii krvotvornýchbuniek
Obnova krvotvorby a imunitného systému je nevyhnutným predpokladom každej úspešnej alogénnejtransplantácie krvotvorných buniek (TKB). Cieľom našej práce bolo sledovanie dynamiky jej obnovyv prvých týždňoch po transplantácii v súbore 36 pacientov po alogénnej TKB. Vzorky periférnejvenóznej krvi sme vyšetrovali v 6-dňových intervaloch, od +16. dňa, až do prepustenia pacienta doambulantnej starostlivosti (+38. deň po TKB). Subpopulácie leukocytov sme vyšetrovali prietokovoucytometriou a cytotoxickú aktivitu NK-buniek sme stanovovali celulárnou fluourescenčnou metódouproti bunkovej línii K562. Cytotoxická aktivita NK-buniek dosiahla hodnoty zdravých jedincov už +22.deň po TKB. Pri porovnávaní súboru pacientov s akútnou chorobou štepu proti hostiteľovi (GvHD) sosúborom pacientov, ktorí na GvHDnetrpeli,sme nezaznamenali štatisticky signifikantné zmeny v počtecelkových leukocytov, lymfocytov, buniek CD3+, CD4+, CD8+, resp. CD56+. Pacienti trpiaci na akútnuGvHD mali však v +22. a +28. dni po TKB štatisticky signifikantne zvýšené percentuálne zastúpenieCD8+ cytotoxických T-lymfocytov v periférnej krvi oproti pacientom bez nej (p = 0,009 a p = 0,03).Podobne sme nezaznamenali ani štatisticky signifikantne zvýšené hodnoty cytotoxickej aktivity NKbuniek.Pacienti po transplantácii kostnej drene však dosahovali počas monitorovania vyššie hodnotycytotoxickej aktivity NK-buniek ako tí, ktorým sa transplantovali krvotvorné bunky získané z periférnejkrvi; tento rozdiel bol štatisticky signifikantný iba v +16. dni po transplantácii.
Klíčová slova:
alogénna transplantácia kostnej drene, transplantácia periférnych krvotvorných buniek,obnova krvotvorby a imunitného sytému, cytotoxická aktivita NK-buniek, akútna Graft versus HostDisease
Authors:
M. Sapák 1; M. Mistrík 2; K. Kozlíková 3; U. W. Schaefer 4; M. Buc 1
Authors‘ workplace:
Imunologický ústav Lekárskej fakulty UK, Bratislava, Slovenská republika 2Klinika hematológie a transfuziológie FN a LF UK, Bratislava, Slovenská republika 3Ústav lekárskej fyziky a biofyziky Lekárskej fakulty UK, Bratislava, Slovenská republika4Klinik fü
1
Published in:
Transfuze Hematol. dnes,, 2004, No. 3, p. 90-98.
Category:
Overview
Successful allogeneic haematopoietic stem cell transplantation (HCT) requires a rapid recovery ofhaematopoiesis and NK-cell cytotoxicity. The aim of our study was to investigate a dynamic of thisrecovery in 35 patients who underwent HCT in the very first weeks after transplantation. Samples ofperipheral blood were investigated in 6 weeks intervals starting from the day +16 until the release ofa patient from hospital to outpatient care (the day +38). Flow cytometry to investigate subpopulationsof leucocytes and nonradioactive Europium release assay using K562 cell line as target cells toappreciate NK-cell cytotoxicity were used. TheNK-cell cytotoxicity reached the level of healthy personson average in the day +22. No statistically significant differences in the recovery of CD3+, CD4+, CD8+and CD56+ cells were observed when the group of transplanted patients suffering from acute graftversus host disease (GvHD) was compared to that with no signs of post-transplant immune reactions.A significantly higher percentage of CD8+ cells were found in the GvHD group of patients on the day+22 (p = 0.009) and +28 (p = 0.03), respectively compared the non-GvHD group of patients; however,differences in the NK-cell cytotoxicity did not reach statistical significance. By comparing recipientsreceiving a bone marrow with those transplanted with peripheral blood stem cells, a statisticallysignificant faster recovery of theNK-cell cytotoxicity was observed on the day +16 after transplantation.
Key words:
blood and immune cells recovery, graft versus host disease, haematopoietic stem celltransplantation, NK-cell cytotoxicity
Labels
Haematology Internal medicine Clinical oncologyArticle was published in
Transfusion and Haematology Today
2004 Issue 3
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