Aminoguanidin podávaný subchronicky intrahipokampálně zlepšuje u diabetických potkanů plnění úkolů pasivního vyhýbání a expresi genů z rodiny Bcl-2

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Cíle:
Pozitivní vliv jednotlivých dávek aminoguanidinu (AG) podaných intrahipokampálně na postižení způsobená diabetem u experimentálního zvířecího modelu diabetu již byl popsán. Cílem této studie bylo zjistit účinky 7denního intrahipokampálního podávání injekcí AG na postižení paměti vyvolané diabetem a jeho roli v apoptóze.

Materiály a metodologie:
72 samců potkanů bylo rozděleno do 9 skupin: kontrolní, kontrolní léčená fyziologickým roztokem, kontrolní léčená AG 10, 30 a 90 μg/potkana, diabetici a diabetici léčení AG 10, 30 a 90 μg/potkana. Následně bylo měřeno zvládání pasivního vyhýbání a pomocí RT-PCR geny z rodiny Bcl-2.

Výsledky:
AG významně snížil kognitivní postižení (počet opakování do osvojení si, latence udržení u step-through testu a doba strávená v zatmaveném oddíle) u diabetických potkanů. Léčba AG navíc významně ovlivnila diabetem vyvolané změny v expresi Bax, Bcl-2 a Bcl-xl.

Závěry:
Sedmidenní intrahipokampální injekční aplikace AG může zlepšit zhoršený kognitivní výkon u diabetických potkanů zvýšením Bcl-2 nebo Bcl-xl a snížením poměrů Bax.

Klíčová slova:
diabetes mellitus – aminoguanidin – hipokampus – osvojení pasivního vyhýbání – rodina genů Bcl-2

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Autoři: M. Alipour ¹; A. Aminabadi ¹; M. Arab-Firouzjaei ²; B. Amini ¹; M. R. Jafari ¹
Působiště autorů: Department of Physiology and Pharmacology, School of Medicine, Zanjān University of Medical Sciences, Zanjān, Iran ¹; Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran ²
Vyšlo v časopise: Cesk Slov Neurol N 2017; 80/113(5): 584-590
Kategorie: Původní práce
prolekare.web.journal.doi_sk: https://doi.org/10.14735/amcsnn2017584

Souhrn

Závěry:
Sedmidenní intrahipokampální injekční aplikace AG může zlepšit zhoršený kognitivní výkon u diabetických potkanů zvýšením Bcl-2 nebo Bcl-xl a snížením poměrů Bax.

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