A divergent CheW confers plasticity to nucleoid-associated chemosensory arrays

English version

Autoři: Annick Guiseppi ^aff001; Juan Jesus Vicente ^aff002; Julien Herrou ^aff001; Deborah Byrne ^aff003; Aurelie Barneoud ^aff001; Audrey Moine ^aff001; Leon Espinosa ^aff001; Marie-Jeanne Basse ^aff004; Virginie Molle ^aff005; Tâm Mignot ^aff001; Philippe Roche ^aff004; Emilia M. F. Mauriello ^aff001
Působiště autorů: Laboratoire de Chimie Bactérienne, Aix Marseille Univ, CNRS, Marseille, France ^aff001; Physiology & Biophysics, University of Washington, Seattle, WA, United States of America ^aff002; Protein Purification Platform, Institut de Microbiologie de la Méditerranée, CNRS, Marseille, France ^aff003; CRCM, Institute Paoli-Calmettes, CNRS, INSERM, Aix Marseille Univ, Marseille, France ^aff004; Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologique, Montpellier II et I University, CNRS, France ^aff005
Vyšlo v časopise: A divergent CheW confers plasticity to nucleoid-associated chemosensory arrays. PLoS Genet 15(12): e32767. doi:10.1371/journal.pgen.1008533
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1008533

Souhrn

Chemosensory systems are highly organized signaling pathways that allow bacteria to adapt to environmental changes. The Frz chemosensory system from M. xanthus possesses two CheW-like proteins, FrzA (the core CheW) and FrzB. We found that FrzB does not interact with FrzE (the cognate CheA) as it lacks the amino acid region responsible for this interaction. FrzB, instead, acts upstream of FrzCD in the regulation of M. xanthus chemotaxis behaviors and activates the Frz pathway by allowing the formation and distribution of multiple chemosensory clusters on the nucleoid. These results, together, show that the lack of the CheA-interacting region in FrzB confers new functions to this small protein.

Klíčová slova:

Crystal structure – Cell motility – Fluorescence microscopy – Histidine – Protein structure – Phosphorylation – Cell fusion – Protein structure comparison

Zdroje

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