ree light chains and pairs of heavy and light chains of immunoglobulins in relation to morbidity of patients before liver transplantation and post-transplantation.
Authors:
A. Jabor 1,2; S. Fraňková 3
; R. Šenkeříková 3; J. Komrsková 1; M. Labanczová 1; D. Viczénová 1; P. Sečník Jr. 3; T. Vacková 1; J. Franeková 1,2
Authors place of work:
Institut klinické a experimentální medicíny, Pracoviště laboratorních metod, Praha, Česká republika
1; Univerzita Karlova, 3. lékařská fakulta, Praha, Česká republika
2; Institut klinické a experimentální medicíny, Klinika hepatogastroenterologie, Praha, Česká republika
3; SK Lab, Lučenec, Slovensko
4
Published in the journal:
Klin. Biochem. Metab., 26, 2018, No. 4, p. 166-172
Summary
Objective:
To assess the role of free light immunoglobulin chains and pairs of heavy and light immunoglobulin chains in patients before and after liver transplantation (LTx). To compare associations of immunoglobulins and their components with comorbidities as assessed by objective laboratory tests and to evaluate development of laboratory tests during 2-year follow-up.
Design:
observational prospective study
Settings:
Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 140 21 Prague
Material and methods:
A group of 174 patients was evaluated before LTx and during a 2-year follow-up. Organ dysfunctions were evaluated by means of ELF score, MELD score, and common laboratory parameters (red blood cells, platelets, liver function, renal function, nutrition, and glucose metabolism). Reagents and analyzers from The Binding Site (SPA Plus and Optilite analyzer), Abbott (Architect ci 16200), Sysmex (XN), Siemens (Centaur CP), and Stago (STA R Max) were used according to the manufacturer´s recommendation. The Spearman correlation, Mann-Whitney, and Friedman tests were used.
Results:
Increased IgA, FLC-κ and FLC-λ before LTx were associated with increased ELF and MELD score, creatinine and uric acid and also with decreased concentrations of hemoglobin, platelets, albumin, prealbumin, lipid parameters, and cholinesterase. In patients with increased concentrations of IgA and FLC-κ before LTx, increased IgA and FLC-κ occurred also during the follow-up and worsening of renal function and lipid metabolism was found.
Conclusion:
We confirmed prognostic role of serum IgA in patients with serious liver diseases requiring transplantation. Similar prognostic role was found for FLC-κ a FLC-λ. The measurement of HLC was not associated with organ dysfunctions and comorbidities in these patients.
Keywords:
liver transplantation, immunoglobulins, free light immunoglobulin chains, pairs of heavy/light immunoglobulin chains, ELF score.
Zdroje
1. Sečník, P. Jr., Lavríková, P., Hošková, L., Malíková, P., Jabor, A., Franeková, J. Free light chain and intact immunoglobulin abnormalities in heart transplant recipients: Two year follow-up timelines and clinical correlations. Transpl Immunol, 2017,41, p. 22-26.
2. Lavríková, P., Sečník, P. Jr., Kubíček, Z., Jabor, A., Hošková, L., Franeková, J. Tacrolimus has immunosuppressive effects on heavy/light chain pairs and freelight chains in patients after heart transplantation: A relationship with infection. Transpl Immunol, 2018, 50, p. 43-47.
3. Inamine, T., Schnabl, B. Immunoglobulin A and liver diseases. J Gastroenterol, 2018, 53, p. 691-700.
4. Hansen, I.S., Hoepel, W., Zaat, S. A. J., Baeten, D. L. P., den Dunnen, J. Serum IgA Immune Complexes Promote Proinflammatory Cytokine Production by Human Macrophages, Monocytes, and Kupffer Cells through FcαRI-TLR Cross-Talk. J Immunol, 2017, 199, p. 4124-4131.
5. Lin, S., Sun, Q., Mao, W., Chen, Y. Serum Immunoglobulin A (IgA) Level Is a Potential Biomarker Indicating Cirrhosis during Chronic Hepatitis B Infection. Gastroenterol Res Pract, 2016, 2016:2495073. doi: 10.1155/2016/2495073.
6. Jabor, A., Kubíček, Z., Fraňková, S., Šenkeříková, R., Franeková, J. Enhanced liver fibrosis (ELF) score: Reference ranges, biological variation in healthy subjects, and analytical considerations. Clin Chim Acta, 2018, 483, p. 291-295.
7. Katzmann, J. A., Willrich, M. A., Kohlhagen, M. C., et al. Monitoring IgA multiple myeloma: immunoglobulin heavy/light chain assays. Clin Chem, 2015, 61, p. 360-367.
8. Ludwig, H., Milosavljevic, D., Berlanga, O. et al. Suppression of the noninvolved pair of the myeloma isotype correlates with poor survival in newly diagnosed and relapsed/refractory patients with myeloma. Am J Hematol, 2016, 91, p. 295-301.
9. Chae, H., Han, E., Yoo, J. et al. Heavy/light chain assay as a biomarker for diagnosis and follow-up of multiple myeloma, Clin Chim Acta, 2018, 479, p. 7-13.
10. Michallet, M., Chapuis-Cellier, C., Dejoie, T. et al. Heavy+light chain monitoring correlates with clinical outcome in multiple myeloma patients. Leukemia, 2018;32:376-382.
11. Sarto, C., Cappellini, F., Giagnacovo, M., Brambilla P. IgMκ-IgMλ pair quantitation in the clinical laboratory practice. Clin Biochem, 2018, 51, p. 56-60.
12. Greil, C., Ihorst, G., Gaiser, F. et al. The serum heavy/light chain immunoassay: A valuable tool for sensitive paraprotein assessment, risk, and disease monitoring in monoclonal gammopathies. Eur J Haematol, 2017, 99, p. 449-458.
13. Jabor, A., Kubíček, Z., Komrsková, J. et al. Within-subject biological variation of pairs of heavy/light immunoglobulin IgM chains (HLC IgM κ and λ) is low and requires monitoring: A comparison with HLC IgA, HLC IgG, and free light immunoglobulin chains (FLC) in healthy subjects. Clin Chim Acta, 2018, 486, p. 311-312.
Štítky
Clinical biochemistry Nuclear medicine Nutritive therapistČlánok vyšiel v časopise
Clinical Biochemistry and Metabolism
2018 Číslo 4
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