Liquid biopsies are approaching routine clinical laboratories
Authors:
B. Friedecký
Authors place of work:
Ústav klinické biochemie a diagnostiky FN Hradec Králové
Published in the journal:
Klin. Biochem. Metab., 28, 2020, No. 3, p. 121-126
Summary
This work is created for education of laboratory professionals in routine clinical laboratories and deals with possibility applying the liquid biopsy in routine mode. The determination of circulating DNA (cfDNA, ctDNA) are main topics of liquid biopsy. Low invasiveness and possibility of often using are big advantages of these tests. Liquid biopsy methods are part of the disruption and digitalization in the of the laboratory medicine. Routine laboratories should be one tool of precision medicine and methods of liquid biopsy, based on genomic (PCR, NGS) methods are surely the part of this process. Important factor for adaptation liquid biopsy to routine work is development of CDx (companion diagnostics tests). Problems of results harmonizing and comparability of these methods are mentioned by means of several cases.
Keywords:
liquid biopsy – Genomics – PCR – NGS – cfDNA – ctDNA – miRNA
Zdroje
1. Mader, S., Pantel, K. Liquid Biopsy. Current status and Future Perspectives. Oncol. Res. Threat., 2017, 40, p. 404-408
2. Tsui, D. W. Y., Blumenthal, G. M., Phillip, R. et al. Development, Validation and Regulatory Considerations for a Liquid Biopsy Test. Clin. Chem., 2020, 66, p. 408-414
3. Sorber, L., Zvaenepoel, K., Jakobs, J. et al. Specialized Blood Collection Tubes for Liquid Biopsy: Improving the Pre-analytical Conditions. Mol. Diagn. Ther., 2019, https://doi. org/10171/s40291-019-00442cx
4. Sorber, L., Zvaenepoel, K., Jakobs, J. et al. Circulation Cell-Free DNA and RNA Analysis in Liquid Biopsy: Optimal Centrifugation Protocols Cancers, 2019 DOI:10. 3390/cancers11040458
5. Faraldi, M., Sansoni, V., Perego, S. et al. Study of preanalytical Variables Affecting the Measurement of Clinically Relevant Free-Circulating microRNA“Focus on Sample Matrix, Platelet Depletion and Storage Conditions. Biochem. Med., (Zagreb) 2019, 30, 010703
6. Ward, G. A., Lenhardt, J., Witte, T. et al. Evaluation of Storage Tubes for Combined Analysis of Circulating Nucleic Acids in Liquid Biopsies, Int. J Mol. Sci., 2019, DOI:10. 3390/ijms20030704
7. Feng, X., Lin, Y., Wan, N. Plasma microRNA detection standardization test. JCCA, 2019, https://doi.org/10.1002/jcla23059
8. Lee, J. S., Kim, M., Seong, M.-W. et al. Plasma vs Serum in circulating tumor DNA measurement: characterization by DNA fragment siting and digital droplet polymerase chain reaction. Clin. Chem. Lab. Med., 2020, 58, p. 527-532
9. Beije, N., Jager, A., Sleijfer, S. Circulation tumor cell enumeration on by the CellSearch syslem. the clinician guide to breast cancer treatment? Cancer Treat. Rev., 2015, 41, p. 144-150
10. Cortés-Hernandez, L. E., Eslani, Z., Pantel, K., Panabinás, C. A. Molecular and Function Characterization of Circulation Tumour Cells: From Discovery to Clinical Application. Clin. Chem., 2019, doi:10. 1373/clinchem-2019. 303586.
11. Siskos, A. P., Pooja, J., Margl-Romisch, W. et al. Inter-laboratory Reproducibility of a Targeted Metabolomics Platform for Analysis of Human Serum and Plasma. Anal. Chem., 2017, 89, p. 656-665
12. AACC Smart Brief 2020 Feb 19. Grail Conducts Interventional Trial of Multi-cancer blood test
13. http://www.dgkl-rfb.de Referenzinstitut fur Bioanqlytic. Ringversuch ctDNA 2019
14. Provencio, M., Peréz-Barrios, C., Barquin, M. et al. Next-generation sequencing for tumor station quantification using liquid biopsies. Clin. Chem. Lab. Med., 2020, https://doi. org/10. 1515/cclm-2019-0745
15. Lampignano, A., Neumann, M. H. D., Weber, S. et al. Multicenter evaluation of Circulating Cell-Free DNA Extraction and Downstream Analyses for the Development of (Pre)analytical Work Flow. Clin. Chem., 2020, 66, p. 149-160
16. Chai, S. Y., Peng, R., Zhang, R. et al. External Qua-lity Assurance of Currentr Technology for the Testing of Cancer-Associated Circulating Free DNA Variants. Pathog. Oncol. Res., 2019, http://doi. org/10. 1007/s12253-019-0074-8
17. Jiang, F., Yang, X., He, X., Yang, M. Circulating DNA, A Potentially Sensitive and Specific Diagnostic Tool for Future Medicine. Dose-Response, 2019, doi.10.1177/1559325819891010
18. Zhao, X., Kang, J., Svetnik, V. et al. A machine learning approach to identify a circulating microRNA signatur for Alzheimer Disease. J Appl. Lab. Med., 2019, doi:10.1373/jalm. 2019. 029595.
19. Faraldi, M., Gamarasca, M., Banfi, G., Lombardi, G. Free Circulating miRNAs Measurement in Clinical Settings:The Still Unsolved Issue of the Normalization. Adv. Clin. Chem., 2018, 87, p. 113-139
20. Donati, S., Ciuffi, S., Brandi, M. L. Human circulating miRNA Real-Time qPCR-based Analysis. An Overview of Endogenous Reference Genes Used for Data Normalization. Int. J Mol. Sci., 2019, doi:10.3390/ijms20184353
21. Tolan, N., Parnas, M. Big data in laboratory medicine. Clin. Chem., 2015, 61, p. 1433-1440.
22. Badrick, T. Banfi, G., Bietenbeck, A. et al. Machine Learning in Clinical Chemists. Clin. Chem., 2019, doi:10.1373/clinchem. 2019.309812
23. Lippi, G., Plebani, M. Integrated diagnostics. the future of Laboratory Medicine? Biochem. Med., (Zagreb) 2020, 30, 010501.
24. Bianchi, D. W., Lamar Parker, R., Werworth, J. et al. DNA sequencing versus standard prenatal aneuploidy screening. N Engl. J Med., 2014, 37, p. 799-808
25. Palomaki, G. E., Kloza, E. M., O´Brien, B. M. et al. The clinical reliability of DNA based screening for fetal aneuploidy screening by primaty obstetrical care providers in the general frequency population. Genet. Med., 2017, 17, p. 778-786
26. Palomaki, G. E., Wyath, P., Glen-Best, R. et al. Assessment of laboratories offering cell free (cf)DNA screening for Down syndrome: results of the 2018-College of American Pathology External Educational Exercises. Genet. Med., 2020, doi:10.1038/s41436-019-0718-4
27. AACC. Ethics of AI and Big Data in Laboratory Medicine. Clin. Lab. News, Jan-Feb 2020
28. Kirwan, J. A., Keddurah-Daouk, R., Mitchell, T. et al. Biobanking for Metabolomic and Lipidomic in Precision Medicine. Clin. Chem., 2019, doi:10.1373/clinchem.2018.298620
29. Baber, R., Kiehntopf, M. Automation in biobanking from a laboratory medicine perspective. J Lab. Med., 2019, 43, p. 329-338
30. Gils, C., Nybo, M. Quality kontrol of preanalytical handling of blood samples for future research: a national survey, J Appl. Lab. Med., 2019, doi:10-1373/jalm.2019.029942
Štítky
Clinical biochemistry Nuclear medicine Nutritive therapistČlánok vyšiel v časopise
Clinical Biochemistry and Metabolism
2020 Číslo 3
Najčítanejšie v tomto čísle
- Laboratory diagnostics of COVID-19
- Vitamin D – prevention and treatment in children and adolescents
- Vitamin D 2019 - standardization and chaos. Miniature model of the presentation of laboratory science at present.
- Vitamin D and cardiovascular disease. What do we know about this in 2020?