What is the Prognostic Importance of Molecular Genetic Factors in Endometrial Carcinoma?

Česká verzia

Authors: E. Křepinská ¹; M. Chmelařová ²; J. Laco ³; V. Palička ²; J. Špaček ¹
Authors place of work: Porodnická a gynekologická klinika, LF UK a FN v Hradci Králové ¹; Ústav klinické biochemie a diagnostiky, LF UK a FN v Hradci Králové ²; Fingerlandův ústav patologie, LF UK a FN v Hradci Králové ³
Published in the journal: Klin Onkol 2012; 25(4): 282-286
Category: Original Articles

Summary

Background:
Evaluation of the importance of molecular genetic factors in endometrial carcinoma based on our review of available literature, and in the case of K- ras mutation based on our own data. The aim of the original part of our study was to compare the presence of K- ras mutation in early stages of endometroid carcinoma with normal endometrium and evaluate the role of the mutation in endometrial carcinogenesis.

Material and methods:
Molecular biological analysis was performed to detect K- ras mutation in samples of endometrial tissue obtained from women treated in the past at the Department of Obstetrics and Gynecology, University Hospital Hradec Kralove. The detection was made from DNA isolated from paraffin embedded sections using K- ras StripAssay™, ViennaLab Diagnostics GmbH.

Results:
K- ras mutation was found in 7 out of 30 specimens of endometroid carcinoma in stage I (23%) and in 3 of 20 specimens of normal endometrium in the control group (15%). K- ras mutations were more frequent in IA stage and grade 1 of endometroid carcinoma.

Conclusion:
The importance of molecular genetic factors in endometrial carcinoma differs depending on the type of carcinoma. In more common type 1 endometroid cancer, published data are not as clear as in type 2 carcinoma, in which prevalence of alteration of p53 reaches 90%. Results of our study performed on local population of women support the theory about the possible role of K- ras mutation as an early event in the process of endometrial carcinogenesis in type 1 tumors.

Key words:
endometrial carcinoma – K- ras gene – mutation

This study was supported by grant of Grant Agency of the Charles University No. 157310.

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manuscript met the ICMJE “uniform requirements” for biomedical papers.

Submitted:
12. 12. 2011

Accepted:
27. 6. 2012

Zdroje

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